Abstract
A virus was isolated from a large cupped narcissus plant which showed yellow stripe symptoms collected in Niigata Prefecture in 1971. The virus was readily transmitted by juice inoculation. Among the test plants of 46 species in 14 families, 37 species in 13 families, namely, Chenopodiaceae, Amaranthaceae, Aizoaceae, Caryophylaceae, Papaveraceae, Cruciferae, Leguminosae, Violaceae, Solanaceae, Pedaliaceae, Cucurbitaceae, Compositae, Amaryllidaceae, were found susceptible to the virus.
The virus was not transmitted byMyzus persicae, but was transmitted by nematode (Xiphinema bakeri). An evidence of soil transmission was provided by greenhouse tests using soil which was infested with nematodes includingXiphinema bakeri. Lots of 25X. bakeri sieved from soil in which infected petunia had been grown for 2 to 3 weeks were placed near the roots of potted healthy petunia. After 6 weeks the virus was detected by juice inoculation and by serological test in all inoculated petunia plants. No infection occurred in control petunia plants grown in soil free fromX. bakeri. The virus was proved to be transmitted also through seeds of soybean.
The virusin vitro withstood heating at 60C for 10 minutes, but not 65C, and 21 days of storage at 22C, but not 28 days. The virus particles were found to be spherical about 25nm in diameter.
Antiserum prepared injecting rabbit with a semi-purified virus preparation showed homologous precipitin tube titre of 1/1024. By agar gel diffusion tests, the virus showed negative reaction to antisera against tomato black ring virus (kindly sent from Dr. B.D. Harrison) and against tobacco ringspot virus and tomato ringspot virus (kindly sent from Dr. R. Stace-Smith), but showed positive reaction to the antiserum against arabis mosaic virus (kindly sent from Dr. B.D. Harrison).
From the above results, the virus was identified as arabis mosaic virus, which has hitherto not been reported in Japan.
Virus-free narcissus seedling juice-inoculated with the virus remained apparently healthy for 15 months, although the virus was recovered from these plants.
