Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens
- PMID:19241134
- DOI: 10.1007/s11248-009-9252-5
Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens
Abstract
Taxadiene synthase gene from Taxus brevifolia was constitutively expressed in the moss Physcomitrella patens using a ubiquitin promoter to produce taxa-4(5),11(12)-diene, the precursor of the anticancer drug paclitaxel. In stable moss transformants, taxa-4(5),11(12)-diene was produced up to 0.05% fresh weight of tissue, without significantly affecting the amounts of the endogenous diterpenoids (ent-kaurene and 16-hydroxykaurane). Unlike higher plants that had been genetically modified to produce taxa-4(5),11(12)-diene, transgenic P. patens did not exhibit growth inhibition due to alteration of diterpenoid metabolic pools. Thus we propose that P. patens is a promising alternative host for the biotechnological production of paclitaxel and its precursors.
Similar articles
- Redirection of carotenoid metabolism for the efficient production of taxadiene [taxa-4(5),11(12)-diene] in transgenic tomato fruit.Kovacs K, Zhang L, Linforth RS, Whittaker B, Hayes CJ, Fray RG.Kovacs K, et al.Transgenic Res. 2007 Feb;16(1):121-6. doi: 10.1007/s11248-006-9039-x. Epub 2006 Nov 15.Transgenic Res. 2007.PMID:17106766
- Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol.Besumbes O, Sauret-Güeto S, Phillips MA, Imperial S, Rodríguez-Concepción M, Boronat A.Besumbes O, et al.Biotechnol Bioeng. 2004 Oct 20;88(2):168-75. doi: 10.1002/bit.20237.Biotechnol Bioeng. 2004.PMID:15449291
- Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene.Hasan MM, Kim HS, Jeon JH, Kim SH, Moon B, Song JY, Shim SH, Baek KH.Hasan MM, et al.Plant Cell Rep. 2014 Jun;33(6):895-904. doi: 10.1007/s00299-014-1568-9. Epub 2014 Jan 25.Plant Cell Rep. 2014.PMID:24463610
- The potential of Physcomitrella patens as a platform for the production of plant-based vaccines.Rosales-Mendoza S, Orellana-Escobedo L, Romero-Maldonado A, Decker EL, Reski R.Rosales-Mendoza S, et al.Expert Rev Vaccines. 2014 Feb;13(2):203-12. doi: 10.1586/14760584.2014.872987. Epub 2014 Jan 3.Expert Rev Vaccines. 2014.PMID:24405402Review.
- Hormonal Diterpenoids Distinct to Gibberellins Regulate Protonema Differentiation in the Moss Physcomitrium patens.Nakajima M, Miyazaki S, Kawaide H.Nakajima M, et al.Plant Cell Physiol. 2020 Dec 23;61(11):1861-1868. doi: 10.1093/pcp/pcaa129.Plant Cell Physiol. 2020.PMID:33057650Review.
Cited by
- From Plant to Yeast-Advances in Biosynthesis of Artemisinin.Zhao L, Zhu Y, Jia H, Han Y, Zheng X, Wang M, Feng W.Zhao L, et al.Molecules. 2022 Oct 14;27(20):6888. doi: 10.3390/molecules27206888.Molecules. 2022.PMID:36296479Free PMC article.Review.
- Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.Zhu X, Liu X, Liu T, Wang Y, Ahmed N, Li Z, Jiang H.Zhu X, et al.Plant Commun. 2021 Aug 9;2(5):100229. doi: 10.1016/j.xplc.2021.100229. eCollection 2021 Sep 13.Plant Commun. 2021.PMID:34746761Free PMC article.Review.
- Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules.Wilson SA, Roberts SC.Wilson SA, et al.Plant Biotechnol J. 2012 Apr;10(3):249-68. doi: 10.1111/j.1467-7652.2011.00664.x. Epub 2011 Nov 8.Plant Biotechnol J. 2012.PMID:22059985Free PMC article.Review.
- Stable heterologous expression of biologically active terpenoids in green plant cells.Ikram NK, Zhan X, Pan XW, King BC, Simonsen HT.Ikram NK, et al.Front Plant Sci. 2015 Mar 18;6:129. doi: 10.3389/fpls.2015.00129. eCollection 2015.Front Plant Sci. 2015.PMID:25852702Free PMC article.Review.
- Exploring optimal Taxol® CYP725A4 activity in Saccharomyces cerevisiae.Nowrouzi B, Lungang L, Rios-Solis L.Nowrouzi B, et al.Microb Cell Fact. 2022 Sep 19;21(1):197. doi: 10.1186/s12934-022-01922-1.Microb Cell Fact. 2022.PMID:36123694Free PMC article.
References
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous