Hippocampal astrocytes exhibit Ca2+-elevating muscarinic cholinergic and histaminergic receptors in situ
- PMID:10646506
- DOI: 10.1046/j.1471-4159.2000.740555.x
Hippocampal astrocytes exhibit Ca2+-elevating muscarinic cholinergic and histaminergic receptors in situ
Abstract
Recent findings suggest that astrocytes respond to neuronally released neurotransmitters with Ca2+ elevations. These Ca2+ elevations may trigger astrocytes to release glutamate, affecting neuronal activity. Neuronal activity is also affected by modulatory neurotransmitters that stimulate G protein-coupled receptors. These neurotransmitters, including acetylcholine and histamine, might affect neuronal activity by triggering Ca2+-dependent release of neurotransmitters from astrocytes. However, there is no physiological evidence for histaminergic or cholinergic receptors on astrocytes in situ. We asked whether astrocytes have these receptors by imaging Ca2+-sensitive dyes sequestered by astrocytes in hippocampal slices. Our results show that immunocytochemically identified astrocytes respond to carbachol and histamine with increases in intracellular free Ca2+ concentration. The H1 histamine receptor antagonist chlorpheniramine inhibited responses to histamine. Similarly, atropine and the M1-selective muscarinic receptor antagonist pirenzepine inhibited carbachol-elicited responses. Astrocyte responses to histamine and carbachol were compared with responses elicited by alpha1-adrenergic and metabotropic glutamate receptor agonists. Individual astrocytes responded to different subsets of receptor agonists. Ca2+ oscillations were the prevalent response pattern only with metabotropic glutamate receptor stimulation. Finally, functional alpha1-adrenergic receptors and muscarinic receptors were not detected before postnatal day 8. Our data show that astrocytes have acetylcholine and histamine receptors coupled to Ca2+. Given that Ca2+ elevations in astrocytes trigger neurotransmitter release, it is possible that these astrocyte receptors modulate neuronal activity.
Similar articles
- Synaptically released acetylcholine evokes Ca2+ elevations in astrocytes in hippocampal slices.Araque A, Martín ED, Perea G, Arellano JI, Buño W.Araque A, et al.J Neurosci. 2002 Apr 1;22(7):2443-50. doi: 10.1523/JNEUROSCI.22-07-02443.2002.J Neurosci. 2002.PMID:11923408Free PMC article.
- GFAP-positive hippocampal astrocytes in situ respond to glutamatergic neuroligands with increases in [Ca2+]i.Porter JT, McCarthy KD.Porter JT, et al.Glia. 1995 Feb;13(2):101-12. doi: 10.1002/glia.440130204.Glia. 1995.PMID:7544323
- Metabotropic receptor-mediated Ca2+ signaling elevates mitochondrial Ca2+ and stimulates oxidative metabolism in hippocampal slice cultures.Kann O, Kovács R, Heinemann U.Kann O, et al.J Neurophysiol. 2003 Aug;90(2):613-21. doi: 10.1152/jn.00042.2003. Epub 2003 Apr 30.J Neurophysiol. 2003.PMID:12724360
- Astrocytes control neuronal excitability in the nucleus accumbens.Fellin T, D'Ascenzo M, Haydon PG.Fellin T, et al.ScientificWorldJournal. 2007 Nov 2;7:89-97. doi: 10.1100/tsw.2007.195.ScientificWorldJournal. 2007.PMID:17982581Free PMC article.Review.
- Role of histaminergic regulation of astrocytes in alcohol use disorder.Riveros ME, Leibold NK, Retamal MA, Ezquer F.Riveros ME, et al.Prog Neuropsychopharmacol Biol Psychiatry. 2024 Jul 13;133:111009. doi: 10.1016/j.pnpbp.2024.111009. Epub 2024 Apr 21.Prog Neuropsychopharmacol Biol Psychiatry. 2024.PMID:38653364Review.
Cited by
- Examination and Estimation of Anticholinergic Burden: Current Trends and Implications for Future Research.Salahudeen MS, Nishtala PS.Salahudeen MS, et al.Drugs Aging. 2016 May;33(5):305-13. doi: 10.1007/s40266-016-0362-5.Drugs Aging. 2016.PMID:27038065
- Somatodendritic targeting of M5 muscarinic receptor in the rat ventral tegmental area: implications for mesolimbic dopamine transmission.Garzón M, Pickel VM.Garzón M, et al.J Comp Neurol. 2013 Sep 1;521(13):2927-46. doi: 10.1002/cne.23323.J Comp Neurol. 2013.PMID:23504804Free PMC article.
- GABA and neuroactive steroid interactions in glia: new roles for old players?Magnaghi V.Magnaghi V.Curr Neuropharmacol. 2007 Mar;5(1):47-64. doi: 10.2174/157015907780077132.Curr Neuropharmacol. 2007.PMID:18615153Free PMC article.
- What is the role of astrocyte calcium in neurophysiology?Agulhon C, Petravicz J, McMullen AB, Sweger EJ, Minton SK, Taves SR, Casper KB, Fiacco TA, McCarthy KD.Agulhon C, et al.Neuron. 2008 Sep 25;59(6):932-46. doi: 10.1016/j.neuron.2008.09.004.Neuron. 2008.PMID:18817732Free PMC article.Review.
- Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex.Oda S, Tsuneoka Y, Yoshida S, Adachi-Akahane S, Ito M, Kuroda M, Funato H.Oda S, et al.J Comp Neurol. 2018 Jun 1;526(8):1329-1350. doi: 10.1002/cne.24409. Epub 2018 Feb 28.J Comp Neurol. 2018.PMID:29424434Free PMC article.
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Miscellaneous