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Deoxyribonucleate Binding and Transformation inRhizobium japonicum

J L Raina1,V V Modi1
1Department of Microbiology, M. S. University of Baroda, Baroda, India
PMCID: PMC251290  PMID:4538250

Abstract

Rhizobium japonicum, capable of binding high-molecular-weight donor32P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native32P-labeled input DNA was bound irreversibly in a temperature-dependent manner. Homologous denatured32P-labeled DNA was incapable of binding to the recipient under similar conditions. CsCl density gradient banding of the donor and recipient DNA indicated homology. The low frequency of transformation could be due to one or more steps that occur between DNA uptake and integration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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