This study generated a transgenic mouse line that enables the in vivo visualization, isolation and transcriptional profiling of oligodendrocyte precursor cells (OPCs) across the lifespan. Analysis of gene-expression changes reveals how OPCs in the cerebral cortex respond to the aging brain environment and highlights pathways that inhibit their differentiation.

Multi-omic analysis of the adult human prostate identifies spatially resolved cell populations with potential links to prostate carcinogenesis.

Advances in long-read sequencing are driving the implementation of these technologies for transcriptome profiling. The authors provide a comprehensive guide to long-read RNA sequencing, including experimental and computational tools, current applications, challenges and opportunities.

Progenitors of intraepithelial T cells (IELps) migrate from the thymus to the intestines after birth where they can develop into unconventional intraepithelial lymphocytes. Here Ocon et al. find that the neuroendocrine peptide hormone somatostatin controls the migration of committed SSTR2-expressing IELps from the thymus to the immature gut.

The authors present a computational framework that leverages mechanical force inference and spatial transcriptomics to enable analyses of the interplay between the transcriptomic and mechanical state.

This study generated a transgenic mouse line that enables the in vivo visualization, isolation and transcriptional profiling of oligodendrocyte precursor cells (OPCs) across the lifespan. Analysis of gene-expression changes reveals how OPCs in the cerebral cortex respond to the aging brain environment and highlights pathways that inhibit their differentiation.

Using a combination of single-cell RNA sequencing, T cell and B cell receptor sequencing, and mass cytometry analyses, we characterized the dynamics of peripheral immune cells across the human lifespan. Based on this resource, we present a single-cell immune aging clock to assess an individual’s immune status across different life stages.

Ayako Suzuki recalls two papers published in the early 2000s by the Functional Annotation of the Mammalian Genome (FANTOM) consortium and the Full-Length Long Japan (FLJ) project, which provided reference catalogues of full-length transcripts in mice and humans, respectively.

In this Tools of the Trade article, Ziye Xu and Yongcheng Wang describe snRandom-seq, a high-throughput, high-sensitivity single-nucleus total RNA sequencing technology optimized for archived samples.

Alzheimer’s disease is a complex, heterogeneous disorder with multiple genetic subtypes. Spatial and single-cell gene expression analyses of these subtypes have provided new insights into general and subtype-specific cellular and molecular mechanisms of Alzheimer’s disease.