Laboratory for Fluorescence Dynamics
A national research center for biomedical fluorescence spectroscopy at the University of California, Irvine
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Abbreviations
- AI: Artificial Intelligence
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FFS: Fluorescence Fluctuation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- MPM: Multiphoton microscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
17th LFD Workshop (2023)
The "17th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 23–27, 2023, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Tentative Agenda and Downloads
Monday, October 23, 2023
- 08:30-09:00 –Breakfast and Introduction by Enrico Gratton.
- 09:00-09:45 –Lecture 1. Michelle Digman: Introduction to Advanced Fluorescence Correlation Spectroscopy (FCS)
- 09:45-10:30 –Lecture 2. Michelle Digman: Raster Image Correlation Spectroscopy (RICS)
- Break
- 11:00-11:45 –Lecture 3. Elizabeth Hinde: Pair-correlation Analyses
- 11:45-12:15 –Lecture 4. Francesco Palomba: Number and Molecular Brightness (N&B) Analysis
- 12:15-12:35 –Application talk 1. Giulia Tedeschi:Fluorescence fluctuation spectroscopy for ion channel studies
- Lunch Break
- 13:30-14:15 –Application talk 2. Falk Schneider:From fluctuations to functions: Exploring bio-molecular organization with FFS
- 14:15-15:00 –Application talk 3. Rodrigo Migueles Ramirez:ICS, TICS and STICS: Image Correlation Spectroscopy, myosin flow and podosome wave propagation
- Break
- 15:30-17:45 –Computer training on FCS, RICS. computing tutorials, software and data files.
- 17:45-18:30 –Application talk 4. TOPTICA: Ultrafast Fiber Lasers and 2P Microscopy: From Theory to Applications (Joseph Mastron)
- 18:30-19:30 –Dinner.
Tuesday, October 24, 2023
- 08:30-09:00 –Breakfast and Introduction by Enrico Gratton.
- 09:00-09:45 –Lecture 5. Elizabeth Hinde: pCOMB: pair-correlation of molecular brightness
- 09:45-10:30 –Lecture 6. Belen Torrado: Fluorescence lifetime and Phasor-FLIM
- Break
- 11:00-11:45 –Lecture 7. Lorenzo Scipioni: Hyperspectral Imaging and Phasor Analysis
- 11:45-12:15 –Application talk 5. Leonel Malacrida: Hyperspectral Imaging of Environmental Sensitive Dyes.
- 12:15-12:35 –Application talk 6. Suman Ranjit: Application of lifetime analysis to tissue samples.
- Lunch Break
- 13:30-14:15 –Application talk 7. ONI: Super-resolution microscopy made easy (Vanessa Lambatan).
- 14:15-15:00 –Demos.
- Break
- 15:30-17:45 –Computer training on FLIM, Hyperspectral Phasors. computing tutorials, software and data files.
- 17:45-18:30 –Special lecture 1. David Jameson: A nano-history of fluorescence.
- 18:30-19:30 –Dinner.
Wednesday, October 25, 2023
- 08:30-09:00 –Breakfast and Introduction by Enrico Gratton.
- 09:00-09:45 –Lecture 8. Belen Torrado: The DIVER microscope for deep tissue imaging
- 09:45-10:30 –Lecture 9. Lorenzo Scipioni: Combining Lifetime and Hyperspectral Detection: Phasor S-FLIM
- Break
- 11:00-11:30 –Lecture 10. Jennifer Prescher: Bioluminescence.
- 11:30-12:15 –Lecture 11. Lorenzo Scipioni: Bioluminescence Phasor Microscope
- 12:15-12:35 –Application talk 8. Lila Halbers and Zachary Torrey: Bioluminescence Phasor Applications.
- Lunch Break
- 13:30-14:15 –Application talk 9. PicoQuant: Quantitative, reproducible Fluorescence Lifetime Imaging for beginners and experts alike (Linnea Olofsson).
- 14:15-15:00 –Laboratory training at LFD facility.
- Break
- 15:30-17:45 –(Cont.)Laboratory training at LFD facility.
- 17:30-18:30 –Application talk 10. Zeiss: Dynamics Profiler: Push Fluorescence Correlation Spectroscopy to a new level (Geert de Vreede).
- 18:45-20:30 –Special Event.
Thursday, October 26, 2023
- 08:30-09:00 –Breakfast and Introduction by Enrico Gratton.
- 09:00-09:45 –Application 11. Francesco Palomba: A new fluorescence method to study protein phase separation
- 09:45-11:00 –Lecutre 12. Alexander Vallmitjana: Clustering and unmixing in the phasor space
- Break
- 11:00-11:45 –Lecture 13. Steve Presse: Photon-by-photon analysis for high resolution fluorescence lifetime maps and species determination.
- 11:45-12:05 –Lecture 14. Mihaela Balu: Instrumentation for in vivo multiphoton microscopy of human skin
- 12:05-12:35 –Application 12. Christoph Gohlke and Leonel Malacrida: Introduction to PhasorPy.
- Lunch Break
- 13:30-14:15 –Computer training on Analysis of single photon arrivals. computing tutorials, software and data files.
- 14:15-15:00 –Laboratory training at LFD facility.
- Break
- 15:30-17:45 –(Cont.)Laboratory training at LFD facility.
- 17:30-18:30 –Application talk 13. Leica: STELLARIS 8 FALCON: Spreading the wings of FLIM (Giulia Ossato).
- 18:30-19:30 –Dinner.
Friday, October 27, 2023
- 08:30-09:00 –Breakfast and Introduction by Enrico Gratton.
- 09:00-10:30 –Data Analysis and Presentation Preparation.
- Break
- 11:00-12:35 –(Cont.)Data Analysis and Presentation Preparation.
- Lunch Break
- 13:30-17:45 –Participants' Symposium and Final Remarks.
Tentative Lecturers
- Mihaela Balu
Beckman Laser Institute, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Christoph Gohlke
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Lila Halbers
The Prescher Lab, University of California, Irvine - Elizabeth Hinde
The University of Melbourne, Australia - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Leonel Malacrida
Institut Pasteur de Montevideo-Universidad de la República del Uruguay - Rodrigo Migueles Ramirez
McGill University, Canada - Suman Ranjit
Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, D.C. - Francesco Palomba
Laboratory for Fluorescence Dynamics, University of California, Irvine - Jennifer Prescher
The Prescher Lab, University of California, Irvine - Steve Presse
The Presse Lab, Arizona State University - Falk Schneider
University of Shouthern California, Los Angeles - Lorenzo Scipioni
Laboratory for Fluorescence Dynamics, University of California, Irvine - Giulia Tedeschi
Laboratory for Fluorescence Dynamics, University of California, Irvine - Belén Torrado
Laboratory for Fluorescence Dynamics, University of California, Irvine - Zachary Torrey
The Prescher Lab, University of California, Irvine - Alexander Vallmitjana
Biomedical Engineering, University of California, Irvine
Tentative Registrants
Laboratory for Fluorescence Dynamics. University of California, Irvine. Department of Biomedical Engineering. 3208 Natural Sciences II. Irvine, CA 92697‑2715, USA. Phone: +1‑949‑824‑7085. Email: lfd@uci.edu. Copyright © 2006‑2025.