›8x52

Electron Microscopy
2.9Å resolution

Cryo-EM structure of human gamma-secretase in complex with Abeta49

Released:
PDB entry 8x52 coloured by chain, front view.
PDB entry 8x52 coloured by chain, side view.
PDB entry 8x52 coloured by chain, top view.
Source organism:Homo sapiens
Related structures:EMD-38059

Function and BiologyDetails

Biochemical function:
Biological process:
Cellular component:

Structure analysisDetails

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-138362 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
C99Chain: E
Molecule details ›
Chain: E
Length: 120 amino acids
Theoretical weight: 13.8 KDa
Source organism:Homo sapiens
Expression system:Homo sapiens
UniProt:
  • Canonical: P05067 (Residues: 671-770; Coverage: 13%)
Gene names:A4,AD1,APP
Sequence domains:
NicastrinChain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 78.47 KDa
Source organism:Homo sapiens
Expression system:Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-709; Coverage: 100%)
Gene names:KIAA0253,NCSTN,UNQ1874/PRO4317
Sequence domains:
Presenilin-1Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.71 KDa
Source organism:Homo sapiens
Expression system:Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names:AD3,PS1,PSEN1,PSNL1
Sequence domains:Presenilin
Gamma-secretase subunit APH-1AChain: C
Molecule details ›
Chain: C
Length: 265 amino acids
Theoretical weight: 29.02 KDa
Source organism:Homo sapiens
Expression system:Homo sapiens
UniProt:
  • Canonical: Q96BI3 (Residues: 1-265; Coverage: 100%)
Gene names:APH1A,CGI-78,PSF,UNQ579/PRO1141
Sequence domains:Aph-1 protein
Gamma-secretase subunit PEN-2Chain: D
Molecule details ›
Chain: D
Length: 101 amino acids
Theoretical weight: 12.04 KDa
Source organism:Homo sapiens
Expression system:Homo sapiens
UniProt:
  • Canonical: Q9NZ42 (Residues: 1-101; Coverage: 100%)
Gene names:MDS033,PEN2,PSENEN
Sequence domains:Presenilin enhancer-2 subunit of gamma secretase

Ligands and Environments

Carbohydrate polymer :NEWComponents:NAG
Carbohydrate polymer
Carbohydrate polymer :NEWComponents:NAG, BMA
Carbohydrate polymer
3 bound ligands:
No modified residues

Experiments and ValidationDetails

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes:EMD-38059
Expression system:Homo sapiens