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multicrispr

This is thereleased version of multicrispr; for the devel version, seemulticrispr.

Multi-locus multi-purpose Crispr/Cas design

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DOI: 10.18129/B9.bioc.multicrispr


Bioconductor version: Release (3.22)

This package is for designing Crispr/Cas9 and Prime Editing experiments. It contains functions to (1) define and transform genomic targets, (2) find spacers (4) count offtarget (mis)matches, and (5) compute Doench2016/2014 targeting efficiency. Care has been taken for multicrispr to scale well towards large target sets, enabling the design of large Crispr/Cas9 libraries.

Author: Aditya Bhagwat [aut, cre], Richie ´Cotton [aut], Rene Wiegandt [ctb], Mette Bentsen [ctb], Jens Preussner [ctb], Michael Lawrence [ctb], Hervé Pagès [ctb], Johannes Graumann [sad], Mario Looso [sad, rth]

Maintainer: Aditya Bhagwat <aditya.bhagwat at uni-marburg.de>

Citation (from within R, entercitation("multicrispr")):

Installation

To install this package, start R (version "4.5") and enter:

if (!require("BiocManager", quietly = TRUE))    install.packages("BiocManager")BiocManager::install("multicrispr")

For older versions of R, please refer to the appropriateBioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("multicrispr")
genome_arithmeticsHTMLR Script
grna_designHTMLR Script
prime_editingHTMLR Script
Reference ManualPDF
NEWSText

Need some help? Ask on the Bioconductor Support site!

Details

biocViewsCRISPR,Software
Version1.20.0
In Bioconductor sinceBioC 3.12 (R-4.0) (5 years)
LicenseGPL-2
DependsR (>= 4.0)
ImportsBiocGenerics,Biostrings,BSgenome,CRISPRseek,data.table,Seqinfo,GenomicFeatures,GenomicRanges,ggplot2, grid,karyoploteR,magrittr, methods, parallel,plyranges,Rbowtie,reticulate,rtracklayer, stats,stringi,tidyr,tidyselect, utils
System Requirements
URLhttps://github.com/bhagwataditya/multicrispr
Bug Reportshttps://github.com/bhagwataditya/multicrispr/issues
See More
SuggestsAnnotationHub,BiocStyle,BSgenome.Hsapiens.UCSC.hg38,BSgenome.Mmusculus.UCSC.mm10,BSgenome.Scerevisiae.UCSC.sacCer1,ensembldb,IRanges,GenomeInfoDb,knitr,magick,rmarkdown,testthat,TxDb.Mmusculus.UCSC.mm10.knownGene
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Build ReportBuild Report

Package Archives

FollowInstallation instructions to use this package in your R session.

Source Packagemulticrispr_1.20.0.tar.gz
Windows Binary (x86_64) multicrispr_1.20.0.zip (64-bit only)
macOS Binary (x86_64)multicrispr_1.20.0.tgz
macOS Binary (arm64)multicrispr_1.20.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/multicrispr
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/multicrispr
Bioc Package Browserhttps://code.bioconductor.org/browse/multicrispr/
Package Short Urlhttps://bioconductor.org/packages/multicrispr/
Package Downloads ReportDownload Stats

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