 | |
 Differentiatedadipocytes in a3T3-L1 cell line stained with Oil Red O | |
| Names | |
|---|---|
| IUPAC name 1-(2,5-dimethyl-4-(2,5-dimethylphenyl) phenyldiazenyl) azonapthalen-2-ol | |
| Identifiers | |
3D model (JSmol) | |
| ChEBI | |
| ChemSpider |
|
| ECHA InfoCard | 100.013.906 |
| MeSH | oil+red+O |
| UNII | |
| |
| |
| Properties | |
| C26H24N4O | |
| Molar mass | 408.49496 |
Except where otherwise noted, data are given for materials in theirstandard state (at 25 °C [77 °F], 100 kPa). | |
Oil Red O (Solvent Red 27,Sudan Red 5B,C.I. 26125, C26H24N4O) is alysochrome (fat-soluble dye)diazo dye used forstaining of neutraltriglycerides andlipids on frozen sections and somelipoproteins on paraffin sections. It has the appearance of a red powder with anabsorbance maximum at 518 nanometers.[1]
Oil Red O is one of the dyes used forSudan staining. Similar dyes includeSudan III,Sudan IV, andSudan Black B. The staining has to be performed on fresh samples, as alcohol fixation removes most lipids. Oil Red O largely replaced Sudan III and Sudan IV, as it provides much deeper red color and the stains are therefore much easier to see.
Oil Red O can be used to mark lipid-containing vacuoles, particularly in cases ofacute lymphoblastic leukemia orBurkitt's lymphoma. It can also be used to stain liver sections for histological analysis, quantify cell lipid content, and to stain the aorta to examine lesions from atherosclerosis.[2]
Inpyrotechnics, Oil Red O is used in some compositions of redcolored smokes.
When staining, Oil Red O can make fat more visible in various cuts in pathology.[3]
It is also used in a technique (the method is called as the dye: Oil Red O), discovered in 2004 byAlexandre Beaudoin, for staining latentfingerprints.[4] This technique allows the development of latent fingerprints on porous exhibits (such as paper, cardboard, etc.) that are dry or wet.
It mainly targets fat deposits on the surface of porous exhibits.[5] It is a non-destructive technique (which does not destroy the exhibit nor prevents the use of other techniques).
It is a safe alternative to the Physical Developer method,[6] and is also used in sequence with other methods of fingerprints development.[7]
