Mycobacterium intermedium
Scientific classification
Domain:	Bacteria
Kingdom:	Bacillati
Phylum:	Actinomycetota
Class:	Actinomycetia
Order:	Mycobacteriales
Family:	Mycobacteriaceae
Genus:	Mycobacterium
Species:	M. intermedium
Binomial name
Mycobacterium intermedium Meier et al. 1993, ATCC 51848

Mycobacterium intermedium is a species of the phylumActinomycetota (Gram-positivebacteria with highguanine and cytosine content, one of the dominant phyla of all bacteria), belonging to the genusMycobacterium.

Etymology: Latin; intermedium, meaning between, rapidly and slowly growingmycobacteria.

Gram-positive, and nonmotileacid-fastcoccobacilli (2.0 μm x 2.6 μm).

Colony characteristics

• Eugonic, smooth andphotochromogenic colonies (3–5mm in diameter) onLöwenstein-Jensen medium.

Physiology

• Growth onLöwenstein-Jensen medium at 22 °C, 31 °C, 37 °C and 41 °C, (optimal temperature between 31 °C and 37 °C), within 2–3 weeks.
• Susceptible toethambutol andrifampin.
• Resistant toisoniazid andstreptomycin.

Differential characteristics

• Phylogenetic position between rapidly and slowly growing mycobacteria.
• M. asiaticum is phenotypically very similar, but can be distinguished by its growth at 22 °C, and by itsdysgonic growth.

• Pulmonary disease
• Biosafety level 2

• Repeatedly isolated from sputum from a patient with pulmonary disease.

Strain 1669/91 = ATCC 51848 = CCUG 37583 = CIP 104542 = DSM 44049 = JCM 13572

• Meier et al. 1993. Mycobacterium intermedium sp. nov. Int. J. Syst. Bacteriol., 1993, 43, 204–209.

• Type strain ofMycobacterium intermedium at BacDive - the Bacterial Diversity Metadatabase

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