Genetic and molecular analysis of fox-1, a numerator element involved in Caenorhabditis elegans primary sex determination
- PMID:9927456
- PMCID: PMC1460491
- DOI: 10.1093/genetics/151.2.617
Genetic and molecular analysis of fox-1, a numerator element involved in Caenorhabditis elegans primary sex determination
Abstract
fox-1 was previously identified as a candidate numerator element based on its overexpression phenotype. FOX-1 is an RRM-type RNA-binding protein, which can bind RNAs in vitro. Western analysis detects FOX-1 throughout development. fox-1::lacZ comes on ubiquitously early during embryogenesis. Postembryonically, fox-1::lacZ is expressed sex specifically in a subset of cells in the head and tail. We describe a Tc1-derived deletion allele [fox-1(Delta)] that removes the RRM domain. fox-1(Delta) confers no phenotype in XXs, but can rescue XO-specific lethality and feminization caused by duplications of the left end of the X. fox-1(Delta) synergizes with putative numerators, resulting in abnormal XX development. Genetic analysis indicated that fox-1(Delta) leads to a slight increase in xol-1 activity, while fox-1(gf) leads to partial loss of xol-1 activity, and xol-1 is epistatic to fox-1. RNase protection experiments revealed increased levels of the 2.2-kb xol-1 message in fox-1(Delta) animals, and reduced levels in fox-1(gf) animals. Additionally, fox-1(Delta) impairs male mating efficiency, which, we propose, represents another function of fox-1, independent of xol-1 and its role in sex determination.
Similar articles
- Dose-dependent action of the RNA binding protein FOX-1 to relay X-chromosome number and determineC. elegans sex.Farboud B, Novak CS, Nicoll M, Quiogue A, Meyer BJ.Farboud B, et al.Elife. 2020 Dec 29;9:e62963. doi: 10.7554/eLife.62963.Elife. 2020.PMID:33372658Free PMC article.
- The primary sex determination signal of Caenorhabditis elegans.Carmi I, Meyer BJ.Carmi I, et al.Genetics. 1999 Jul;152(3):999-1015. doi: 10.1093/genetics/152.3.999.Genetics. 1999.PMID:10388819Free PMC article.
- Molecular antagonism between X-chromosome and autosome signals determines nematode sex.Farboud B, Nix P, Jow MM, Gladden JM, Meyer BJ.Farboud B, et al.Genes Dev. 2013 May 15;27(10):1159-78. doi: 10.1101/gad.217026.113. Epub 2013 May 10.Genes Dev. 2013.PMID:23666922Free PMC article.
- Sex in the wormcounting and compensating X-chromosome dose.Meyer BJ.Meyer BJ.Trends Genet. 2000 Jun;16(6):247-53. doi: 10.1016/s0168-9525(00)02004-7.Trends Genet. 2000.PMID:10827451Review.
- Developmental genetics of Caenorhabditis elegans sex determination.Kuwabara PE.Kuwabara PE.Curr Top Dev Biol. 1999;41:99-132. doi: 10.1016/s0070-2153(08)60271-9.Curr Top Dev Biol. 1999.PMID:9784974Review.
Cited by
- Mechanisms of sex determination and X-chromosome dosage compensation.Meyer BJ.Meyer BJ.Genetics. 2022 Feb 4;220(2):iyab197. doi: 10.1093/genetics/iyab197.Genetics. 2022.PMID:35100381Free PMC article.Review.
- Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals.Underwood JG, Boutz PL, Dougherty JD, Stoilov P, Black DL.Underwood JG, et al.Mol Cell Biol. 2005 Nov;25(22):10005-16. doi: 10.1128/MCB.25.22.10005-10016.2005.Mol Cell Biol. 2005.PMID:16260614Free PMC article.
- The worm solution: a chromosome-full of condensin helps gene expression go down.Csankovszki G, Petty EL, Collette KS.Csankovszki G, et al.Chromosome Res. 2009;17(5):621-35. doi: 10.1007/s10577-009-9061-y.Chromosome Res. 2009.PMID:19802703Free PMC article.
- A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG.Jin Y, Suzuki H, Maegawa S, Endo H, Sugano S, Hashimoto K, Yasuda K, Inoue K.Jin Y, et al.EMBO J. 2003 Feb 17;22(4):905-12. doi: 10.1093/emboj/cdg089.EMBO J. 2003.PMID:12574126Free PMC article.
- Sex and Mitonuclear Adaptation in ExperimentalCaenorhabditis elegans Populations.Wernick RI, Christy SF, Howe DK, Sullins JA, Ramirez JF, Sare M, Penley MJ, Morran LT, Denver DR, Estes S.Wernick RI, et al.Genetics. 2019 Mar;211(3):1045-1058. doi: 10.1534/genetics.119.301935. Epub 2019 Jan 22.Genetics. 2019.PMID:30670540Free PMC article.
References
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Molecular Biology Databases