A serum shock induces circadian gene expression in mammalian tissue culture cells
- PMID:9635423
- DOI: 10.1016/s0092-8674(00)81199-x
A serum shock induces circadian gene expression in mammalian tissue culture cells
Abstract
The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2 (rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erb alpha, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus.
Comment in
- Why the rat-1 fibroblast should replace the SCN as the in vitro model of choice.Rosbash M.Rosbash M.Cell. 1998 Jun 12;93(6):917-9. doi: 10.1016/s0092-8674(00)81197-6.Cell. 1998.PMID:9635421Review.No abstract available.
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