Human cytotrophoblast differentiation/invasion is abnormal in pre-eclampsia
- PMID:9403732
- PMCID: PMC1858365
Human cytotrophoblast differentiation/invasion is abnormal in pre-eclampsia
Abstract
During human placental development, cytotrophoblast stem cells differentiate and invade the uterus. Simultaneously, the cells modulate their expression of several classes of stage-specific antigens that mark transitions in the differentiation process and play a role in either uterine invasion (integrin cell-extracellular matrix receptors and matrix metalloproteinase-9) or immune interactions (HLA-G). The pregnancy disease pre-eclampsia is associated with shallow cytotrophoblast invasion. Previously we showed, by immunofluorescence localization on placental tissue, that in pre-eclampsia invasive cytotrophoblasts fail to properly modulate their integrin repertoire. This finding suggests possible abnormalities in the differentiation pathway that leads to uterine invasion. Here we used a culture system that supports this differentiation process to compare the differentiative and invasive potential of cytotrophoblasts obtained from control (n = 8, 22 to 38 weeks) and pre-eclamptic (n = 9, 24 to 38 weeks) placentas. In culture, the cells from pre-eclamptic placentas failed to properly modulate alpha1 integrin and matrix metalloproteinase-9 expression at the protein and mRNA levels. Their invasive potential was also greatly reduced. Likewise, the cells failed to up-regulate HLA-G protein and mRNA expression. These results suggest that defective cytotrophoblast differentiation/invasion can have significant consequences to the outcome of human pregnancy (ie, development of pre-eclampsia) and that, by the time delivery becomes necessary, the defect is not reversed by removing the cells from the maternal environment.
Similar articles
- Hypoxia alters early gestation human cytotrophoblast differentiation/invasion in vitro and models the placental defects that occur in preeclampsia.Genbacev O, Joslin R, Damsky CH, Polliotti BM, Fisher SJ.Genbacev O, et al.J Clin Invest. 1996 Jan 15;97(2):540-50. doi: 10.1172/JCI118447.J Clin Invest. 1996.PMID:8567979Free PMC article.
- Extracellular matrix composition and hypoxia regulate the expression of HLA-G and integrins in a human trophoblast cell line.Kilburn BA, Wang J, Duniec-Dmuchowski ZM, Leach RE, Romero R, Armant DR.Kilburn BA, et al.Biol Reprod. 2000 Mar;62(3):739-47. doi: 10.1095/biolreprod62.3.739.Biol Reprod. 2000.PMID:10684818
- Human placental HLA-G expression is restricted to differentiated cytotrophoblasts.McMaster MT, Librach CL, Zhou Y, Lim KH, Janatpour MJ, DeMars R, Kovats S, Damsky C, Fisher SJ.McMaster MT, et al.J Immunol. 1995 Apr 15;154(8):3771-8.J Immunol. 1995.PMID:7706718
- Why is placentation abnormal in preeclampsia?Fisher SJ.Fisher SJ.Am J Obstet Gynecol. 2015 Oct;213(4 Suppl):S115-22. doi: 10.1016/j.ajog.2015.08.042.Am J Obstet Gynecol. 2015.PMID:26428489Free PMC article.Review.
- Human cytotrophoblast invasion.Fisher SJ, Damsky CH.Fisher SJ, et al.Semin Cell Biol. 1993 Jun;4(3):183-8. doi: 10.1006/scel.1993.1022.Semin Cell Biol. 1993.PMID:8347835Review.
Cited by
- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening.Pu Y, Veiga-Lopez A.Pu Y, et al.Methods Mol Biol. 2024;2728:223-234. doi: 10.1007/978-1-0716-3495-0_18.Methods Mol Biol. 2024.PMID:38019404Free PMC article.
- Myeloid and lymphoid dendritic cells in normal pregnancy and pre-eclampsia.Darmochwal-Kolarz D, Rolinski J, Tabarkiewicz J, Leszczynska-Gorzelak B, Buczkowski J, Wojas K, Oleszczuk J.Darmochwal-Kolarz D, et al.Clin Exp Immunol. 2003 May;132(2):339-44. doi: 10.1046/j.1365-2249.2003.02136.x.Clin Exp Immunol. 2003.PMID:12699426Free PMC article.
- Integrated Proteomic and N-Glycoproteomic Profiling of Placental Tissues of Patients with Preeclampsia.Wang H, Shi Y, Ma J, Wang W, Gao J, Zhao L, Zhao T, Ding G.Wang H, et al.Int J Womens Health. 2023 Jan 13;15:59-68. doi: 10.2147/IJWH.S387672. eCollection 2023.Int J Womens Health. 2023.PMID:36660462Free PMC article.
- Transforming growth factor-β signaling governs the differentiation program of extravillous trophoblasts in the developing human placenta.Haider S, Lackner AI, Dietrich B, Kunihs V, Haslinger P, Meinhardt G, Maxian T, Saleh L, Fiala C, Pollheimer J, Latos PA, Knöfler M.Haider S, et al.Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2120667119. doi: 10.1073/pnas.2120667119. Epub 2022 Jul 6.Proc Natl Acad Sci U S A. 2022.PMID:35867736Free PMC article.
- Umbilical Vein and Maternal Serum Inhibin A, Activin A, and Follistatin Concentrations in IUGR due to Placental Dysfunction Pregnancies.Kasam S, Shen Z, Cao H, Sudeep MV.Kasam S, et al.J Obstet Gynaecol India. 2013 Mar;63(1):26-31. doi: 10.1007/s13224-012-0190-4. Epub 2013 Apr 16.J Obstet Gynaecol India. 2013.PMID:24431596Free PMC article.
References
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials