doi: 10.1073/pnas.94.8.3823.
Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
Affiliations
- PMID:9108062
- PMCID: PMC20525
- DOI: 10.1073/pnas.94.8.3823
Item in Clipboard
Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
P D Keightley et al. Proc Natl Acad Sci U S A..
Display options
Format
Display options
Format
Abstract
Theory concerning the evolution of sex and recombination and mutation load relies on information on rates and distributions of effects of deleterious mutations. Direct information on the genomic mutation rate in Drosophila implies that an accumulation of mildly deleterious mutations reduces viability of populations by at least 1% per generation. We carried out an experiment to measure the deleterious mutation rate in Caenorhabditis elegans, in which independent sublines were maintained with one hermaphrodite parent per generation, conditions that minimize the opportunity for natural selection and lead to random fixation of deleterious mutations. After 60 generations of mutation accumulation, negligible changes in mean reproductive output and lifespan occurred, but the genetic variance increased at rates typical for life history traits in other species. The estimated deleterious mutation rate per haploid genome for fitness, U, was 0.0026, a figure two orders of magnitude smaller than previously measured for viability in Drosophila.
Figures
Fixation probability of a new mutation occurring in a self-fertilizing line plotted against its selection coefficients. The fitnesses of the wild-type, heterozygote, and mutant genotypes were 1, 1 − hs, and 1 − s, respectively. For the case of selfing, it can be shown that the function relating fixation probability (u) ands isu = (1 − s)/(2 − s), and the mean time to fixation or loss is three generations. Fixation probability is independent ofh, the degree of dominance of the mutation.
Mean number of worms produced per hermaphrodite in the MA lines and the between-line component of variance of productivity estimated by ANOVA. (Bar = 1 SE.)
Distributions of mean productivities of 48 mutation accumulation lines from generation 60 and 40 replicates from generation 0. gen, generation.
Proportions of worms surviving each day for the MA lines from generations 0, 32, and 60. Mean lifespan for generations 0, 32, and 60 was 14.0, 14.0, and 13.4 days, respectively. Gen, generation.
Similar articles
- The fitness effects of spontaneous mutations in Caenorhabditis elegans.Vassilieva LL, Hook AM, Lynch M.Vassilieva LL, et al.Evolution. 2000 Aug;54(4):1234-46. doi: 10.1111/j.0014-3820.2000.tb00557.x.Evolution. 2000.PMID:11005291
- Low impact of germline transposition on the rate of mildly deleterious mutation in Caenorhabditis elegans.Bégin M, Schoen DJ.Bégin M, et al.Genetics. 2006 Dec;174(4):2129-36. doi: 10.1534/genetics.106.065508. Epub 2006 Oct 22.Genetics. 2006.PMID:17057249Free PMC article.
- Fitness decline in spontaneous mutation accumulation lines of Caenorhabditis elegans with varying effective population sizes.Katju V, Packard LB, Bu L, Keightley PD, Bergthorsson U.Katju V, et al.Evolution. 2015 Jan;69(1):104-16. doi: 10.1111/evo.12554. Epub 2014 Dec 9.Evolution. 2015.PMID:25338758
- Terumi Mukai and the riddle of deleterious mutation rates.Keightley PD, Eyre-Walker A.Keightley PD, et al.Genetics. 1999 Oct;153(2):515-23. doi: 10.1093/genetics/153.2.515.Genetics. 1999.PMID:10511536Free PMC article.Review.No abstract available.
- Causes of natural variation in fitness: evidence from studies of Drosophila populations.Charlesworth B.Charlesworth B.Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):1662-9. doi: 10.1073/pnas.1423275112. Epub 2015 Jan 8.Proc Natl Acad Sci U S A. 2015.PMID:25572964Free PMC article.Review.
Cited by
- The effect of spontaneous mutations on competitive ability.Schaack S, Allen DE, Latta LC 4th, Morgan KK, Lynch M.Schaack S, et al.J Evol Biol. 2013 Feb;26(2):451-6. doi: 10.1111/jeb.12058. Epub 2012 Dec 17.J Evol Biol. 2013.PMID:23252614Free PMC article.
- Distribution of fitness effects caused by single-nucleotide substitutions in bacteriophage f1.Peris JB, Davis P, Cuevas JM, Nebot MR, Sanjuán R.Peris JB, et al.Genetics. 2010 Jun;185(2):603-9. doi: 10.1534/genetics.110.115162. Epub 2010 Apr 9.Genetics. 2010.PMID:20382832Free PMC article.
- Direct estimate of the mutation rate and the distribution of fitness effects in the yeast Saccharomyces cerevisiae.Wloch DM, Szafraniec K, Borts RH, Korona R.Wloch DM, et al.Genetics. 2001 Oct;159(2):441-52. doi: 10.1093/genetics/159.2.441.Genetics. 2001.PMID:11606524Free PMC article.
- Inbreeding load, average dominance and the mutation rate for mildly deleterious alleles in Mimulus guttatus.Willis JH.Willis JH.Genetics. 1999 Dec;153(4):1885-98. doi: 10.1093/genetics/153.4.1885.Genetics. 1999.PMID:10581293Free PMC article.
- Estimates of the rate and distribution of fitness effects of spontaneous mutation in Saccharomyces cerevisiae.Zeyl C, DeVisser JA.Zeyl C, et al.Genetics. 2001 Jan;157(1):53-61. doi: 10.1093/genetics/157.1.53.Genetics. 2001.PMID:11139491Free PMC article.
References
- Kondrashov A S. Nature (London) 1988;336:435–440. - PubMed
- Charlesworth B. Curr Biol. 1996;6:149–162. - PubMed
- Barton N H, Turelli M. Annu Rev Genet. 1989;23:337–370. - PubMed
- Nordborg M, Charlesworth B, Charlesworth D. Genet Res. 1996;67:159–174. - PubMed
- Crow J F. Oxford Surv Evol Biol. 1993;9:3–42.
Publication types
MeSH terms
Related information
LinkOut - more resources
Full Text Sources