doi: 10.1073/pnas.94.8.3823.
Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
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- PMID:9108062
- PMCID: PMC20525
- DOI: 10.1073/pnas.94.8.3823
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Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
P D Keightley et al. Proc Natl Acad Sci U S A..
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Abstract
Theory concerning the evolution of sex and recombination and mutation load relies on information on rates and distributions of effects of deleterious mutations. Direct information on the genomic mutation rate in Drosophila implies that an accumulation of mildly deleterious mutations reduces viability of populations by at least 1% per generation. We carried out an experiment to measure the deleterious mutation rate in Caenorhabditis elegans, in which independent sublines were maintained with one hermaphrodite parent per generation, conditions that minimize the opportunity for natural selection and lead to random fixation of deleterious mutations. After 60 generations of mutation accumulation, negligible changes in mean reproductive output and lifespan occurred, but the genetic variance increased at rates typical for life history traits in other species. The estimated deleterious mutation rate per haploid genome for fitness, U, was 0.0026, a figure two orders of magnitude smaller than previously measured for viability in Drosophila.
Figures
Fixation probability of a new mutation occurring in a self-fertilizing line plotted against its selection coefficients. The fitnesses of the wild-type, heterozygote, and mutant genotypes were 1, 1 − hs, and 1 − s, respectively. For the case of selfing, it can be shown that the function relating fixation probability (u) ands isu = (1 − s)/(2 − s), and the mean time to fixation or loss is three generations. Fixation probability is independent ofh, the degree of dominance of the mutation.
Mean number of worms produced per hermaphrodite in the MA lines and the between-line component of variance of productivity estimated by ANOVA. (Bar = 1 SE.)
Distributions of mean productivities of 48 mutation accumulation lines from generation 60 and 40 replicates from generation 0. gen, generation.
Proportions of worms surviving each day for the MA lines from generations 0, 32, and 60. Mean lifespan for generations 0, 32, and 60 was 14.0, 14.0, and 13.4 days, respectively. Gen, generation.
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