doi: 10.1016/0378-1119(96)00085-6.
Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe
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- PMID:8890754
- DOI: 10.1016/0378-1119(96)00085-6
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Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe
R Ohi et al. Gene..
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Abstract
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
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