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.1979 Jan;76(1):106-10.
doi: 10.1073/pnas.76.1.106.

Expression in Escherichia coli of chemically synthesized genes for human insulin

Expression in Escherichia coli of chemically synthesized genes for human insulin

D V Goeddel et al. Proc Natl Acad Sci U S A.1979 Jan.

Abstract

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. Complete purification of the A chain and partial purification of the B chain were achieved. These products were mixed, reduced, and reoxidized. The presence of insulin was detected by radioimmunoassay.

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References

    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3900-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 - PubMed
    1. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5765-9 - PubMed

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