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.1993 Oct 18;332(3):226-8.
doi: 10.1016/0014-5793(93)80637-a.

Mass spectrometric evidence for a disulfide bond in aequorin regeneration

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Mass spectrometric evidence for a disulfide bond in aequorin regeneration

Y Ohmiya et al. FEBS Lett..
Free article

Abstract

Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys145 and Cys152 and that the reduction of this bond is involved in the regeneration of aequorin.

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