Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells
- PMID:8391465
- DOI: 10.1006/excr.1993.1182
Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells
Abstract
The presence of sperm cells characterized by increased sensitivity of DNA in situ to denaturation and by abnormal morphology (shape) in semen is associated with low fertility. Such cells often appear in increasing numbers following exposure to toxic or mutagenic agents. In the present study, the sensitivity of DNA in situ to acid-induced denaturation was assayed in sperm cells of 25 patients attending a human fertility center. The same samples were also subjected to analysis of DNA strand breaks. A new method for detecting DNA strand breaks in situ by labeling 3'-OH termini with biotinylated dUTP in a reaction employing exogenous terminal deoxynucleotidyl transferase has been applied. Both methods utilized flow cytometry. A good correlation was observed in all samples between the percentage of sperm cells with DNA strand breaks and the percentage of cells characterized by an increased sensitivity of DNA to denaturation (r = 0.87; P < = 0.05). It is likely, thus, that the sperm cells characterized by increased DNA sensitivity to denaturation have extensive DNA strand breakage. The above properties of abnormal sperm cells, which in all probability are dead in terms of their reproductive capacity, resemble properties of apoptotic somatic cells. Activation of the endogenous endonuclease which causes extensive DNA breakage, thus, is characteristic to both the programmed death of somatic cells (apoptosis) and functional elimination (of possibly defective) germ cells from the reproductive pool.
Similar articles
- Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay.Sailer BL, Jost LK, Evenson DP.Sailer BL, et al.J Androl. 1995 Jan-Feb;16(1):80-7.J Androl. 1995.PMID:7768756
- Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA.Gorczyca W, Tuziak T, Kram A, Melamed MR, Darzynkiewicz Z.Gorczyca W, et al.Cytometry. 1994 Feb 1;15(2):169-75. doi: 10.1002/cyto.990150211.Cytometry. 1994.PMID:8168404
- Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays.Gorczyca W, Gong J, Darzynkiewicz Z.Gorczyca W, et al.Cancer Res. 1993 Apr 15;53(8):1945-51.Cancer Res. 1993.PMID:8467513
- Flow cytometric evaluation of sperm parameters in relation to fertility potential.Gillan L, Evans G, Maxwell WM.Gillan L, et al.Theriogenology. 2005 Jan 15;63(2):445-57. doi: 10.1016/j.theriogenology.2004.09.024.Theriogenology. 2005.PMID:15626410Review.
- Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).Evenson DP, Wixon R.Evenson DP, et al.Toxicol Appl Pharmacol. 2005 Sep 1;207(2 Suppl):532-7. doi: 10.1016/j.taap.2005.03.021.Toxicol Appl Pharmacol. 2005.PMID:15987647Review.
Cited by
- Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?Mohammadi Z, Tavalaee M, Gharagozloo P, Drevet JR, Nasr-Esfahani MH.Mohammadi Z, et al.Basic Clin Androl. 2020 Aug 13;30:12. doi: 10.1186/s12610-020-00110-8. eCollection 2020.Basic Clin Androl. 2020.PMID:32817794Free PMC article.
- Silane-coated silica particle colloid processing of human sperm.Perez SM, Chan PJ, Patton WC, King A.Perez SM, et al.J Assist Reprod Genet. 1997 Aug;14(7):388-93. doi: 10.1007/BF02766146.J Assist Reprod Genet. 1997.PMID:9285323Free PMC article.
- TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation.Ribeiro SC, Muratori M, De Geyter M, De Geyter C.Ribeiro SC, et al.PLoS One. 2017 Aug 7;12(8):e0181802. doi: 10.1371/journal.pone.0181802. eCollection 2017.PLoS One. 2017.PMID:28787000Free PMC article.
- Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro.Jung IH, Yeon KH, Song HR, Hwang YS.Jung IH, et al.Clin Exp Dent Res. 2020 Dec;6(6):669-676. doi: 10.1002/cre2.321. Epub 2020 Aug 2.Clin Exp Dent Res. 2020.PMID:32743893Free PMC article.
- Electroejaculated baboon (Papio anubis) sperm requires a higher dosage of pentoxifylline to enhance motility.Cseh S, Chan PJ, Corselli J, Bailey LL.Cseh S, et al.J Assist Reprod Genet. 2000 Sep;17(8):449-53. doi: 10.1023/a:1009469319596.J Assist Reprod Genet. 2000.PMID:11062856Free PMC article.
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources