The byssus of the zebra mussel, Dreissena polymorpha. II: Structure and polymorphism of byssal polyphenolic protein families
- PMID:8180628
The byssus of the zebra mussel, Dreissena polymorpha. II: Structure and polymorphism of byssal polyphenolic protein families
Abstract
The byssus of the zebra mussel Dreissena polymorpha is the key element of its adhesive strategy. It consists of a bundle of threads tipped by adhesive plaques and attached to the mussel at the base of its byssal-synthesizing organ, the foot. Two polyphenolic protein precursors of the byssus have been purified from the foot. These precursors, Dpfp-1 and Dpfp-2 (Dreissena polymorpha foot protein), with apparent M(r) values of 76 and 26 K, respectively, contain 3,4-dihydroxyphenylalanine (DOPA) integrated into their primary sequence, but differ from previously characterized polyphenolic proteins from marine mussels. The related quagga mussel (Dreissena spp.?) has homologous proteins with significantly different compositions. The zebra mussel DOPA proteins are tandemly repetitive with unique oligopeptide motif sequences, contain tryptophan, and are O-glycosylated primarily on threonine residues. Galactosamine was the only carbohydrate detected after hydrolysis. Dpfp-1 constitutes a polymorphic family of polypeptides with, unusually, an acidic range of pI values between 5.3 and 6.5. The detection of carbohydrate in the thread and in the juncture between thread and plaque suggests that these two proteins are localized in those regions where they may function as lacquers or structural elements.
Similar articles
- The byssus of the zebra mussel, Dreissena polymorpha. I: Morphology and in situ protein processing during maturation.Rzepecki LM, Waite JH.Rzepecki LM, et al.Mol Mar Biol Biotechnol. 1993 Oct;2(5):255-66.Mol Mar Biol Biotechnol. 1993.PMID:8180627
- Byssal proteins of the freshwater zebra mussel, Dreissena polymorpha.Gantayet A, Ohana L, Sone ED.Gantayet A, et al.Biofouling. 2013;29(1):77-85. doi: 10.1080/08927014.2012.746672.Biofouling. 2013.PMID:23211030
- Development of a cDNA microarray of zebra mussel (Dreissena polymorpha) foot and its use in understanding the early stage of underwater adhesion.Xu W, Faisal M.Xu W, et al.Gene. 2009 May 1;436(1-2):71-80. doi: 10.1016/j.gene.2009.01.007. Epub 2009 Jan 23.Gene. 2009.PMID:19393183
- Wresting the muscle from mussel beards: research and applications.Rzepecki LM, Waite JH.Rzepecki LM, et al.Mol Mar Biol Biotechnol. 1995 Dec;4(4):313-22.Mol Mar Biol Biotechnol. 1995.PMID:8541982Review.
- Mussel Byssus Structure-Function and Fabrication as Inspiration for Biotechnological Production of Advanced Materials.Harrington MJ, Jehle F, Priemel T.Harrington MJ, et al.Biotechnol J. 2018 Dec;13(12):e1800133. doi: 10.1002/biot.201800133. Epub 2018 Aug 24.Biotechnol J. 2018.PMID:30076756Review.
Cited by
- Understanding marine mussel adhesion.Silverman HG, Roberto FF.Silverman HG, et al.Mar Biotechnol (NY). 2007 Nov-Dec;9(6):661-81. doi: 10.1007/s10126-007-9053-x. Epub 2007 Nov 8.Mar Biotechnol (NY). 2007.PMID:17990038Free PMC article.Review.
- Factorial microarray analysis of zebra mussel (Dreissena polymorpha: Dreissenidae, Bivalvia) adhesion.Xu W, Faisal M.Xu W, et al.BMC Genomics. 2010 May 28;11:341. doi: 10.1186/1471-2164-11-341.BMC Genomics. 2010.PMID:20509938Free PMC article.
- Novel proteins identified in the insoluble byssal matrix of the freshwater zebra mussel.Gantayet A, Rees DJ, Sone ED.Gantayet A, et al.Mar Biotechnol (NY). 2014 Apr;16(2):144-55. doi: 10.1007/s10126-013-9537-9. Epub 2013 Sep 24.Mar Biotechnol (NY). 2014.PMID:24057171
- Fingerprinting of Proteins that Mediate Quagga Mussel Adhesion using a De Novo Assembled Foot Transcriptome.Rees DJ, Hanifi A, Obille A, Alexander R, Sone ED.Rees DJ, et al.Sci Rep. 2019 Apr 19;9(1):6305. doi: 10.1038/s41598-019-41976-7.Sci Rep. 2019.PMID:31004089Free PMC article.
- Invasive mussels fashion silk-like byssus via mechanical processing of massive horizontally acquired coiled coils.Simmons M, Horbelt N, Sverko T, Scoppola E, Jackson DJ, Harrington MJ.Simmons M, et al.Proc Natl Acad Sci U S A. 2023 Nov 28;120(48):e2311901120. doi: 10.1073/pnas.2311901120. Epub 2023 Nov 20.Proc Natl Acad Sci U S A. 2023.PMID:37983489Free PMC article.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Research Materials
Miscellaneous