doi: 10.1002/rcm.1290080913.
Detection of 500-nucleotide DNA by laser desorption mass spectrometry
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- PMID:7949335
- DOI: 10.1002/rcm.1290080913
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Detection of 500-nucleotide DNA by laser desorption mass spectrometry
K Tang et al. Rapid Commun Mass Spectrom.1994 Sep.
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Abstract
We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.
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