doi: 10.1093/jat/18.7.385.
Benzodiazepine screening using EMIT II and TDx: urine hydrolysis pretreatment required
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- PMID:7861751
- DOI: 10.1093/jat/18.7.385
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Benzodiazepine screening using EMIT II and TDx: urine hydrolysis pretreatment required
R Meatherall. J Anal Toxicol.1994 Nov-Dec.
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Abstract
Urine specimens were collected from individuals prescribed oral doses of the following benzodiazepine tablets: diazepam, oxazepam, temazepam, lorazepam, alprazolam, flurazepam, and chlordiazepoxide. An aliquot was hydrolyzed using Helix pomatia beta-glucuronidase. Both the hydrolyzed and unhydrolyzed urine pairs were subjected to EMIT II and TDx immunoassay screening tests and to gas chromatographic-mass spectrometric quantitation. Hydrolysis is required to ensure adequate detection of oxazepam, temazepam, and lorazepam with either screening method. A 100-ng/mL cutoff is required when screening for lorazepam, following therapeutic doses. The EMIT II is more sensitive than the TDx as a screening test for benzodiazepines.
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