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Comparative Study
.1994 Sep 1;23(1):272-4.
doi: 10.1006/geno.1994.1493.

Localization of the human beta-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development

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Comparative Study

Localization of the human beta-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development

C Kraus et al. Genomics..

Abstract

The human beta-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of beta-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of beta-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21.

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