Purification of an MCM-containing complex as a component of the DNA replication licensing system
- PMID:7760937
- DOI: 10.1038/375418a0
Purification of an MCM-containing complex as a component of the DNA replication licensing system
Abstract
Replication licensing factor (RLF) ensures that eukaryotic chromosomal DNA is replicated exactly once in each cell cycle. On exit from metaphase, RLF is activated and binds to or modifies chromatin. This modification (the 'licence') is required for subsequent DNA replication; the licence is also inactivated in the process of replication. Active RLF is not imported into the nucleus, so further DNA replication cannot occur until the DNA is relicensed by passage throught mitosis. We have developed an assay to purify RLF from Xenopus eggs. Activity resolves into two components, RLF-M and RLF-B, both of which are required for licensing. RLF-M has been purified to apparent homogeneity: it consists of three polypeptides, one of which is a Xenopus homologue of the yeast MCM3 protein. Xenopus Mcm3 associates with chomatin in G1 and is removed during replication, consistent with its being a component of the RLF system.
Comment in
- Cell cycle. A licence to replicate.Huberman JA.Huberman JA.Nature. 1995 Jun 1;375(6530):360-1. doi: 10.1038/375360a0.Nature. 1995.PMID:7760926No abstract available.
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