Selective vagal rhizotomies: a new dorsal surgical approach used for intestinal deafferentations
- PMID:7503320
- DOI: 10.1152/ajpregu.1995.269.5.R1279
Selective vagal rhizotomies: a new dorsal surgical approach used for intestinal deafferentations
Abstract
We have developed a dorsal intracranial surgery that is minimally invasive and gives excellent access to either afferent or efferent vagal rootlets to produce selective deafferentations or deefferentations in the rat. We have combined this new unilateral afferent rhizotomy with a contralateral celiac branch cut (to completely deafferent the intestines) and a duodenal catheter placement 4 cm distal to the pylorus. Animals were maintained with 17 h/day access to a nutritionally complete liquid diet. Measures of first meal size, daily intake, and body weight before and after both surgeries indicated that animals with unilateral vagal deafferentiations recovered as fast and completely as sham-operated controls. Intraduodenal oleate (1.2 kcal) infusions reduced the size of the first meal in surgical controls (by 64%; P < 0.01) but not in the deafferented rats. A dual wheat germ agglutinin-horseradish peroxidase/Fluorogold protocol provides verification of sensory and motor lesions. The selective vagal deafferentation provided by the new surgery offers a useful model for determining gastrointestinal sites of nutrient detection and separating pre- and postabsorptive consequences of a meal.
Similar articles
- A method for selective section of vagal afferent or efferent axons in the rat.Norgren R, Smith GP.Norgren R, et al.Am J Physiol. 1994 Oct;267(4 Pt 2):R1136-41. doi: 10.1152/ajpregu.1994.267.4.R1136.Am J Physiol. 1994.PMID:7524370
- Afferent innervation of gastrointestinal tract smooth muscle by the hepatic branch of the vagus.Phillips RJ, Baronowsky EA, Powley TL.Phillips RJ, et al.J Comp Neurol. 1997 Jul 28;384(2):248-70.J Comp Neurol. 1997.PMID:9215721
- Gastric volume detection after selective vagotomies in rats.Phillips RJ, Powley TL.Phillips RJ, et al.Am J Physiol. 1998 Jun;274(6):R1626-38. doi: 10.1152/ajpregu.1998.274.6.R1626.Am J Physiol. 1998.PMID:9608017
- Suppression of meal size by intestinal nutrients is eliminated by celiac vagal deafferentation.Walls EK, Phillips RJ, Wang FB, Holst MC, Powley TL.Walls EK, et al.Am J Physiol. 1995 Dec;269(6 Pt 2):R1410-9. doi: 10.1152/ajpregu.1995.269.6.R1410.Am J Physiol. 1995.PMID:8594944
- Abdominal vagal afferent pathways and their distributions of intraganglionic laminar endings in the rat duodenum.Wang FB, Young YK, Kao CK.Wang FB, et al.J Comp Neurol. 2012 Apr 1;520(5):1098-113. doi: 10.1002/cne.22812.J Comp Neurol. 2012.PMID:22102316
Cited by
- Factors regulating vagal sensory development: potential role in obesities of developmental origin.Fox EA, Murphy MC.Fox EA, et al.Physiol Behav. 2008 Apr 22;94(1):90-104. doi: 10.1016/j.physbeh.2007.11.024. Epub 2007 Nov 22.Physiol Behav. 2008.PMID:18234244Free PMC article.
- Central mechanisms mediating the hypophagic effects of oleoylethanolamide and N-acylphosphatidylethanolamines: different lipid signals?Romano A, Tempesta B, Provensi G, Passani MB, Gaetani S.Romano A, et al.Front Pharmacol. 2015 Jun 26;6:137. doi: 10.3389/fphar.2015.00137. eCollection 2015.Front Pharmacol. 2015.PMID:26167152Free PMC article.Review.
- Thoracic cross-over pathways of the rat vagal trunks.Horn CC, Friedman MI.Horn CC, et al.Brain Res. 2005 Oct 26;1060(1-2):153-61. doi: 10.1016/j.brainres.2005.08.037. Epub 2005 Sep 28.Brain Res. 2005.PMID:16197931Free PMC article.
- Vagal branches involved in inhibition of bradykinin-induced synovial plasma extravasation by intrathecal nicotine and noxious stimulation in the rat.Miao FJ, Jänig W, Levine JD.Miao FJ, et al.J Physiol. 1997 Jan 15;498 ( Pt 2)(Pt 2):473-81. doi: 10.1113/jphysiol.1997.sp021873.J Physiol. 1997.PMID:9032694Free PMC article.
- Effects of cholecystokinin-8s in the nucleus tractus solitarius of vagally deafferented rats.Baptista V, Browning KN, Travagli RA.Baptista V, et al.Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1092-100. doi: 10.1152/ajpregu.00517.2006. Epub 2006 Nov 22.Am J Physiol Regul Integr Comp Physiol. 2007.PMID:17122331Free PMC article.
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources