Stress fiber sarcomeres of fibroblasts are contractile
- PMID:6893813
- DOI: 10.1016/0092-8674(80)90365-7
Stress fiber sarcomeres of fibroblasts are contractile
Abstract
Living cultured fibroblasts were microinjected with rhodamine-labeled smooth muscle alpha-actinin and visualized by video-intensified fluorescence microscopy. The alpha-actinin incorporated into the stress fibers and exhibited a regularly striped arrangement. The fluorescently labeled stress fibers remained intact despite glycerol or digitonin extraction of the cells; furthermore, these cell models contracted upon addition of MgATP. During this process, sections of alpha-actinin-labeled stress fibers contracted up to 25%; shortening proceeded in the nonfluorescent part of the stress fiber sarcomeres. In glycerol-extracted cell models, adenylylimidodiphosphate, ADP and pyrophosphate inhibited but vanadate, N-ethylmaleimide-modified heavy meromyosin, cytochalasin B, colchicine, phalloidin and DNAase I did not. Cytochalasin B was inhibitory when added to the intact cells before glycerol extraction. These morphological and biochemical findings demonstrate that stress fiber sarcomeres of fibroblasts are contractile elements and support the concept that an actomyosin system may be involved.
Similar articles
- Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin.Sanger JM, Mittal B, Pochapin MB, Sanger JW.Sanger JM, et al.J Cell Biol. 1986 Jun;102(6):2053-66. doi: 10.1083/jcb.102.6.2053.J Cell Biol. 1986.PMID:2423530Free PMC article.
- Interaction of fluorescently-labeled contractile proteins with the cytoskeleton in cell models.Sanger JW, Mittal B, Sanger JM.Sanger JW, et al.J Cell Biol. 1984 Sep;99(3):918-28. doi: 10.1083/jcb.99.3.918.J Cell Biol. 1984.PMID:6540785Free PMC article.
- In vivo distribution and turnover of fluorescently labeled actin microinjected into human fibroblasts.Kreis TE, Winterhalter KH, Birchmeier W.Kreis TE, et al.Proc Natl Acad Sci U S A. 1979 Aug;76(8):3814-8. doi: 10.1073/pnas.76.8.3814.Proc Natl Acad Sci U S A. 1979.PMID:291042Free PMC article.
- Organisations of actin and fibroblast locomotion.Small JV.Small JV.Prog Clin Biol Res. 1982;85 Pt B:341-58.Prog Clin Biol Res. 1982.PMID:6750631Review.No abstract available.
- Dynamic changes in chromaffin cell cytoskeleton as prelude to exocytosis.Trifaró JM, Rodríguez del Castillo A, Vitale ML.Trifaró JM, et al.Mol Neurobiol. 1992 Winter;6(4):339-58. doi: 10.1007/BF02757940.Mol Neurobiol. 1992.PMID:1337454Review.
Cited by
- Association of microinjected myosin and its subfragments with myofibrils in living muscle cells.Johnson CS, McKenna NM, Wang Y.Johnson CS, et al.J Cell Biol. 1988 Dec;107(6 Pt 1):2213-21. doi: 10.1083/jcb.107.6.2213.J Cell Biol. 1988.PMID:3058721Free PMC article.
- Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of alpha-actinin.Pavalko FM, Burridge K.Pavalko FM, et al.J Cell Biol. 1991 Aug;114(3):481-91. doi: 10.1083/jcb.114.3.481.J Cell Biol. 1991.PMID:1907287Free PMC article.
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.Bildyug N.Bildyug N.Int J Mol Sci. 2019 Oct 11;20(20):5054. doi: 10.3390/ijms20205054.Int J Mol Sci. 2019.PMID:31614676Free PMC article.Review.
- Dynamics of the cytoskeleton in Amoeba proteus. I. Redistribution of microinjected fluorescein-labeled actin during locomotion, immobilization and phagocytosis.Stockem W, Hoffmann HU, Gruber B.Stockem W, et al.Cell Tissue Res. 1983;232(1):79-96. doi: 10.1007/BF00222375.Cell Tissue Res. 1983.PMID:6683994
- Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility.Keshavarz M, Tan B, Venkatakrishnan K.Keshavarz M, et al.Sci Rep. 2016 Oct 19;6:35425. doi: 10.1038/srep35425.Sci Rep. 2016.PMID:27759054Free PMC article.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources