doi: 10.3109/02713688408997211.
Dipeptidyl peptidase III of human cataractous lenses. Partial purification
- PMID:6368131
- DOI: 10.3109/02713688408997211
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Dipeptidyl peptidase III of human cataractous lenses. Partial purification
A A Swanson et al. Curr Eye Res.1984 Feb.
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Abstract
A partial purification of dipeptidyl peptidase III has been achieved from human cataractous lens. The specific activity was increased 45.5-fold over that of the original aqueous extract. The exopeptidase exhibited a marked preference for the release of Arg-Arg from Arg-Arg-2-NNap at the optimum pH 8.8 and 37 degrees. The Km for this substrate was estimated to be 6.061 X 10(-3). Lens DPP III was inhibited by EDTA, p-chloromercuriphenyl sulfonate, puromycin and DFP. The preparation contained leucyl aminopeptidase and a neutral endopeptidase as contaminating proteases.
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