Mammalian Antigen Display for Pandemic Countermeasures
- PMID:38315367
- DOI: 10.1007/978-1-0716-3666-4_12
Mammalian Antigen Display for Pandemic Countermeasures
Abstract
Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen structure-function landscapes, and the surveillance of emerging viral lineages. Cell surface display of a viral antigen or its subdomains can facilitate these goals by coupling the phenotypes of protein variants to their DNA sequence. Screening surface-displayed proteins via flow cytometry also eliminates time-consuming protein purification steps. Prior approaches have primarily relied on yeast as a display chassis. However, yeast often cannot express large viral glycoproteins, requiring their truncation into subdomains. Here, we describe a method to design and express antigens on the surface of mammalian HEK293T cells. We discuss three use cases, including screening of stabilizing mutations, deep mutational scanning, and epitope mapping. The mammalian antigen display platform described herein will accelerate ongoing and future pandemic countermeasures.
Keywords: Antigen; Coronavirus; Flow cytometry; Hemagglutinin; Influenza; Spike; Surface display.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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References
- Marani M, Katul GG, Pan WK, Parolari AJ (2021) Intensity and frequency of extreme novel epidemics. Proc Natl Acad Sci 118(35):e2105482118.https://doi.org/10.1073/pnas.2105482118 - DOI - PubMed - PMC
- Weldon WC, Wang B-Z, Martin MP, Koutsonanos DG, Skountzou I, Compans RW (2010) Enhanced immunogenicity of stabilized trimeric soluble influenza hemagglutinin. PLoS One 5(9):e12466.https://doi.org/10.1371/journal.pone.0012466 - DOI - PubMed - PMC
- Sanders RW et al (2013) A next-generation cleaved, soluble HIV-1 env trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodies. PLoS Pathog 9(9):e1003618.https://doi.org/10.1371/journal.ppat.1003618 - DOI - PubMed - PMC
- Lu Y, Welsh JP, Swartz JR (2013) Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. Proc Natl Acad Sci 111(1):125–130.https://doi.org/10.1073/pnas.1308701110 - DOI - PubMed - PMC
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