doi: 10.1126/science.abp9444. Epub 2022 Sep 2.
Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration
Xiaoyu Wei # 1 2, Sulei Fu # 3 4, Hanbo Li # 2 5 6, Yang Liu # 2, Shuai Wang # 2 7, Weimin Feng # 2 7, Yunzhi Yang # 8, Xiawei Liu 5, Yan-Yun Zeng 3 4, Mengnan Cheng 2 7, Yiwei Lai 9, Xiaojie Qiu 10 11, Liang Wu 2 7, Nannan Zhang 5, Yujia Jiang 2 8, Jiangshan Xu 2 7, Xiaoshan Su 5, Cheng Peng 3 4, Lei Han 2 12 13, Wilson Pak-Kin Lou 3 4, Chuanyu Liu 2 13, Yue Yuan 2 7, Kailong Ma 2, Tao Yang 2, Xiangyu Pan 3, Shang Gao 5, Ao Chen 2 14, Miguel A Esteban 9 15, Huanming Yang 2 16, Jian Wang 2 16, Guangyi Fan 2, Longqi Liu 1 2 7 13, Liang Chen 17, Xun Xu 2 18, Ji-Feng Fei 3, Ying Gu 1 2 7 18
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- PMID:36048929
- DOI: 10.1126/science.abp9444
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Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration
Xiaoyu Wei et al. Science..
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Abstract
The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron-like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.
Comment in
- A mosaic of new and old cell types.Faltine-Gonzalez DZ, Kebschull JM.Faltine-Gonzalez DZ, et al.Science. 2022 Sep 2;377(6610):1043-1044. doi: 10.1126/science.add9465. Epub 2022 Sep 1.Science. 2022.PMID:36048955
- Mapping vertebrate brain evolution.Minton K.Minton K.Nat Rev Genet. 2022 Nov;23(11):647. doi: 10.1038/s41576-022-00535-z.Nat Rev Genet. 2022.PMID:36167907No abstract available.
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