Movatterモバイル変換

[0]ホーム
URL:

Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
Thehttps:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

NIH NLM Logo
Log inShow account info
Access keysNCBI HomepageMyNCBI HomepageMain ContentMain Navigation
pubmed logo
Advanced Clipboard
User Guide

Full text links

BioMed Central full text link BioMed Central Free PMC article
Full text links

Actions

Share

.2022 May 14;23(1):369.
doi: 10.1186/s12864-022-08599-1.

2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress

Affiliations

2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress

Fengkun Lu et al. BMC Genomics..

Abstract

Background: Drought stress is the most limiting factor for plant growth and crop production worldwide. As a major cereal crop, wheat is susceptible to drought. Thus, discovering and utilizing drought-tolerant gene resources from related species are highly important for improving wheat drought resistance. In this study, the drought tolerance of wheat Zhongmai 8601-Thinopyrum intermedium 7XL/7DS translocation line YW642 was estimated under drought stress, and then two-dimensional difference gel electrophoresis (2D-DIGE) based proteome analysis of the developing grains was performed to uncover the drought-resistant proteins.

Results: The results showed that 7XL/7DS translocation possessed a better drought-tolerance compared to Zhongmai 8601. 2D-DIGE identified 146 differential accumulation protein (DAP) spots corresponding to 113 unique proteins during five grain developmental stages of YW642 under drought stress. Among them, 55 DAP spots corresponding to 48 unique proteins displayed an upregulated expression, which were mainly involved in stress/defense, energy metabolism, starch metabolism, protein metabolism/folding and transport. The cis-acting element analysis revealed that abundant stress-related elements were present in the promoter regions of the drought-responsive protein genes, which could play important roles in drought defense. RNA-seq and RT-qPCR analyses revealed that some regulated DAP genes also showed a high expression level in response to drought stress.

Conclusions: Our results indicated that Wheat-Th. intermedium 7XL/7DS translocation line carried abundant drought-resistant proteins that had potential application values for wheat drought tolerance improvement.

Keywords: 2D-DIGE; 7XL/7DS translocation; Drought tolerance; Grain proteome; Wheat.

© 2022. The Author(s).

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Performance of Zhongmai 8601 and wheat-Thinopyron intermedium 7XL/7DS translocation line (YW642) under drought stress
Fig. 2
Fig. 2
Identification of the upregulated drought-responsive differentially accumulated protein (DAP) spots in YW642 during drought stress. 2D-DIGE image exhibited 55 DAP spots during grain development at 20 days after flowering
Fig. 3
Fig. 3
Number of differential accumulation protein (DAP) spots from 15, 20, 25, 30 and 45 DPA in wheat-Thinopyrum intermedium 7XL/7DS translocation line YW642 under drought stress. Five Venn diagrams represent the number of protein spots in the control and YW642 under drought stress group at five different grain developmental stages. The up and down arrows of the overlapping part represent the upregulated expression and downregulated expression in the corresponding period. Blue circle represents Zhongmai 8601 under drought stress while the red represents YW642 under drought stress
Fig. 4
Fig. 4
Functional classification and subcellular location of the 55 upregulated drought-responsive DAPs identified in the 7XL/7DS translocation line YW642.A Function classification of the 146 drought-responsive proteins in YW642 under drought stress.B Subcellular localization prediction of the 146 drought-responsive proteins in YW642 under drought stress.C Function classification of the 55 upregulated drought-responsive proteins in YW642 under drought stress.D Subcellular localization prediction of the 55 upregulated drought-responsive proteins in YW642 under drought stress.E Subcellular localization assay via Arabidopsis protoplast cells. GFP: GFP fluorescence signal. Green fluorescence indicates the location of DAPs; mCherry: red fluorescent dye; Red fluorescent signal indicates the location of chloroplasts in protoplasts; Bright light: field of bright light; Merged: emergence of the GFP fluorescence signal, mChery red fluorescent signal and bright light field; Control: 16318-35S-GFP empty vector. Scale bar = 10 μm
Fig. 5
Fig. 5
Dynamic accumulation profiling of the 55 upregulated drought-responsive DAPs identified in the 7XL/7DS translocation line YW642. Cluster analysis of the 55 DAPs identified in wheat-Thinopyrum intermedium 7XL/7DS translocation line during different grain development stages under drought stress. Each column stands for samples from different treatments and five grain development stages (15, 20, 25, 30, 45 days post anthesis, DPA). Each row uses a color ratio based on the relative ratio to display the changes in DAP spot, blue means low expression, red means high expression and yellow means the middle
Fig. 6
Fig. 6
Protein-protein interaction (PPI) networks of the 25 drought-responsive DAPs identified in the 7XL/7DS translocation line YW642. KOG2099: Alpha-1,4 glucan phosphorylase (spot 15); KOG1335: Dihydrolipoyl dehydrogenase (spot 23); KOG0710: SHSP domain-containing protein (spot 49); KOG2392: Serpin-Z2B (spot 59); PER1: Peroxidase 1 (spot 65); KOG0258: Aminotran_1_2 domain-containing protein (spot 87); KOG1643: Triosephosphate isomerase (spot 102); KOG0069: Formate dehydrogenase (spot 105); KOG1184: Pyruvate decarboxylase (spot 122); KOG4742: PR4B (spot 128); KOG0175: Proteasome subunit beta (spot 131); TSA: 2-Cys peroxiredoxin BAS1 (spot 135); KOG0460: Elongation factor Tu (spot 138); KOG2638: UTP-glucose-1-phosphate uridylyltransferase (spot 139); KOG1350: ATP synthase beta subunit (spot 140); KOG2943: Glyoxalase I (spot 145)
Fig. 7
Fig. 7
RNA-seq expression profiles of the 26 drought-responsive DAP genes in the 7XL/7DS translocation line YW642 under various biotic and abiotic stresses. The number in bracket is the DAP spot number
Fig. 8
Fig. 8
RT-qPCR analysis of nine representative DAP genes from 7XL/7DS translocation line YW642 during grain development in response to drought stress.AGPL, ADP-glucose pyrophos-phorylase large subunit;enolase, enolase coding gene;TSA, 2-Cys peroxiredoxin BAS1;BADH, betaine-aldehyde dehydrogenase;atp2, ATP synthase beta subunit;rbcS, ribulose-1,5-bisp-hosphate carboxylase/oxygenase small subunit;DHAR, dehydroascorbate reductase;BDAI, Dimeric alpha-amylase inhibitor. Statistically significant differences are calculated based on an independent Student’s t-tests: *p < 0.05; **p < 0.01
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Shewry PR, Hey SJ. The contribution of wheat to human diet and health. Food Energy Secur. 2015;4(3):178–202. doi: 10.1002/fes3.64. - DOI - PMC - PubMed
    1. Zörb C, Langenkämper G, Betsche T, Niehaus K, Barsch A. Metabolite profiling of wheat grains (Triticum aestivum L.) from organic and conventional agriculture. J Agric Food Chem. 2006;54(21):8301–8306. doi: 10.1021/jf0615451. - DOI - PubMed
    1. Asseng S, Martre P, Maiorano A, Rotter RP, O’leary G J, Fitzgerald GJ, Girousse C, Motzo R, Giunta F, Babar MA, et al: Climate change impact and adaptation for wheat protein. Glob Chang Biol. 2019;25(1):155-173. - PubMed
    1. Lesk C, Rowhani P, Ramankutty N. Influence of extreme weather disasters on global crop production. Nature. 2016;529(7584):84–87. doi: 10.1038/nature16467. - DOI - PubMed
    1. Ray DK, Gerber JS, MacDonald GK, West PC. Climate variation explains a third of global crop yield variability. Nat Commun. 2015;6:5989. doi: 10.1038/ncomms6989. - DOI - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources

Full text links
BioMed Central full text link BioMed Central Free PMC article
Cite
Send To

NCBI Literature Resources

MeSHPMCBookshelfDisclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

[8]ページ先頭
©2009-2025 Movatter.jp