doi: 10.1126/science.abh4049. Epub 2021 Sep 23.
Cell-free chemoenzymatic starch synthesis from carbon dioxide
Tao Cai 1 2, Hongbing Sun 1 2, Jing Qiao 1 2, Leilei Zhu 2 3, Fan Zhang 1 2, Jie Zhang 2 3, Zijing Tang 2 3, Xinlei Wei 2 3, Jiangang Yang 2 3, Qianqian Yuan 2 4, Wangyin Wang 5, Xue Yang 2 4, Huanyu Chu 2 4, Qian Wang 2 4, Chun You 2 3, Hongwu Ma 2 4, Yuanxia Sun 2 3, Yin Li 1 2, Can Li 5, Huifeng Jiang 2 4, Qinhong Wang 1 2 4, Yanhe Ma 1 2 3
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- PMID:34554807
- DOI: 10.1126/science.abh4049
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Cell-free chemoenzymatic starch synthesis from carbon dioxide
Tao Cai et al. Science..
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Abstract
Starches, a storage form of carbohydrates, are a major source of calories in the human diet and a primary feedstock for bioindustry. We report a chemical-biochemical hybrid pathway for starch synthesis from carbon dioxide (CO2) and hydrogen in a cell-free system. The artificial starch anabolic pathway (ASAP), consisting of 11 core reactions, was drafted by computational pathway design, established through modular assembly and substitution, and optimized by protein engineering of three bottleneck-associated enzymes. In a chemoenzymatic system with spatial and temporal segregation, ASAP, driven by hydrogen, converts CO2 to starch at a rate of 22 nanomoles of CO2 per minute per milligram of total catalyst, an ~8.5-fold higher rate than starch synthesis in maize. This approach opens the way toward future chemo-biohybrid starch synthesis from CO2.
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