doi: 10.1007/BF00268260.
Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector
- PMID:345098
- DOI: 10.1007/BF00268260
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Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector
R M Teather et al. Mol Gen Genet..
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Abstract
The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions where lactose carrier protein comprises 1.4% of the cytoplasmic membrane protein.
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