Increasing ovarian NAD+ levels improve mitochondrial functions and reverse ovarian aging
- PMID:32492457
- DOI: 10.1016/j.freeradbiomed.2020.05.003
Increasing ovarian NAD+ levels improve mitochondrial functions and reverse ovarian aging
Erratum in
- Corrigendum to "Increasing ovarian NAD+ levels improve mitochondrial functions and reverse ovarian aging" [Free Rad. Biol. Med. 156C (2020) 1-10].Yang Q, Cong L, Wang Y, Luo X, Li H, Wang H, Zhu J, Dai S, Jin H, Yao G, Shi S, Hsueh AJ, Sun Y.Yang Q, et al.Free Radic Biol Med. 2022 Feb 1;179:433-434. doi: 10.1016/j.freeradbiomed.2021.04.014. Epub 2021 Apr 23.Free Radic Biol Med. 2022.PMID:33902947No abstract available.
Abstract
Loss of follicles together with decreased oocyte quality and quantity contribute to age-associated ovarian senescence and infertility. Although underlying mechanisms for ovarian senescence are still unknown, mitochondrial dysfunctions have been reported. Here, we showed age-dependent decreases in ovarian Nicotinamide Adenine Dinucleotide (NAD+) levels in mice whereas supplementing aging mice with nicotinamide riboside (NR), an NAD+ precursor, increased ovarian NAD+ content. We found that increases in ovarian NAD+ levels in aging mice led to increased number of ovarian follicles and ovulatory potential as well as increased live birth rate. NR supplementation also reduced levels of reactive oxygen species and decreased spindle anomalies in aging oocytes, together with increased mitochondrial membrane potential (ΔΨm) and decreased mitochondrial clustering. In addition, NR supplementation improved ovarian mitochondrial energy metabolism. Our data suggested that supplementation with NAD+ precursors in vivo and in vitro could be potential therapeutic approaches for treating age-related ovarian infertility.
Keywords: Infertility; Mitochondria; NAD(+); Ovary aging.
Copyright © 2020 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of competing interest There is none of conflict of interest to declare.
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