doi: 10.1002/ijc.2910350513.
N-ras gene activation in the RD human rhabdomyosarcoma cell line
- PMID:3158613
- DOI: 10.1002/ijc.2910350513
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N-ras gene activation in the RD human rhabdomyosarcoma cell line
P Chardin et al. Int J Cancer..
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Abstract
A transforming N-ras allele was molecularly cloned from the RD human rhabdomyosarcoma cell line, and the nature of its activation studied. Construction of chimeric recombinants between the RD-transforming allele and a normal human allele enabled us to localize the alteration responsible for the activation to the second exon of the N-ras gene. The nucleotide sequence of this exon, when compared to that of the normal allele, revealed a single difference at the 61st amino acid position of the encoded protein; the CAA codon for glutamine in the normal allele was mutated to a CAT codon for histidine in the RD-transforming allele. This result is the first description of a histidine replacing glutamine in the 61st position and provides further evidence that the 61st amino acid is one of the preferential sites for N-ras activation.
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