Movatterモバイル変換

[0]ホーム
URL:

Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
Thehttps:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

NIH NLM Logo
Log inShow account info
Access keysNCBI HomepageMyNCBI HomepageMain ContentMain Navigation
pubmed logo
Advanced Clipboard
User Guide

Full text links

MDPI full text link MDPI Free PMC article
Full text links

Actions

Share

.2018 Nov 21;23(11):3039.
doi: 10.3390/molecules23113039.

Coptisine Suppresses Mast Cell Degranulation and Ovalbumin-Induced Allergic Rhinitis

Affiliations

Coptisine Suppresses Mast Cell Degranulation and Ovalbumin-Induced Allergic Rhinitis

Shuilian Fu et al. Molecules..

Abstract

Coptisine is one of the main components of isoquinoline alkaloids in the coptidis rhizome. The effect of coptisine on allergic rhinitis has not been investigated. In this study, we report the effects and mechanisms of coptisine using monoclonal anti-2,4,6-dinitrophenyl-immunoglobulin (Ig) E/human serum albumin (DNP-IgE/HSA)-stimulated rat basophilic leukemia cells (RBL-2H3 cells) in vitro and an ovalbumin (OVA)-induced allergic rhinitis (AR) in mice. The results showed that coptisine markedly decreased the levels of β-hexosaminidase, histamine, interleukin (IL)-4, and tumor necrosis factor (TNF)-α. Coptisine also prevented morphological changes, such as restoring an elongated shape, inhibiting granule release on toluidine blue staining, and reorganizing inhibited filamentous actins (F-actin). Additionally, coptisine blocked the phosphorylation of phosphoinositide3-kinase (PI3K)/Akt (as known as protein kinase B(PKB)) in RBL-2H3 cell. Furthermore, the results showed that coptisine suppressed OVA-induced allergic rhinitis symptoms, such as nasal rubbing and OVA-specific IgE, and histamine, IL-4 and TNF-α levels in the serum of AR mice. These data suggested that coptisine should have inhibitory effects on the inflammatory responses of mast cells, and may be beneficial for the development of coptisine as a potential anti-allergic drug.

Keywords: allergic rhinitis; coptisine; mast cell.

PubMed Disclaimer

Conflict of interest statement

All authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effect of coptisine on the viability of RBL-2H3 cells. All data were expressed as the mean ± SE.
Figure 2
Figure 2
Effect of coptisine on the levels of β-hexosaminidase and histamine from mast cells. Coptisine pretreated (30, 20 or 10 μM) in DNP-IgE/HSA sensitized RBL-2H3 cells. (A) The level of β-hexosaminidase; (B) The level of histamine (the absolute concentration of histamine for control is 210.64 ng/mL). All data were expressed as the mean ± SE. **p < 0.01, ***p < 0.001, in comparison with control group; ###p < 0.001 in comparison with control group.
Figure 3
Figure 3
Effect of coptisine on IL-4, TNF-α levels in DNP-IgE/HSA-stimulated RBL-2H3 cells. Coptisine pretreated (30, 20 or 10 μM) in DNP-IgE/HSA sensitized RBL-2H3 cells. (A) The level of IL-4; (B) The level of TNF-α. All data were expressed as the mean ± SE. *p < 0.05, **p < 0.01, ***p < 0.001, in comparison with DNP-IgE/HSA group; ###p < 0.001 in comparison with control group.
Figure 4
Figure 4
Effects of coptisine with toluidine blue staining in DNP-IgE/HSA-sensitised cells. (A) Normal RBL-2H3 cells; (B) DNP-IgE/HSA-sensitised RBL-2H3 cells; (C) ketotifen fumarate-pretreated RBL-2H3 cells sensitized with DNP-IgE/HSA; (D) coptisine (30 μM)-pretreated RBL-2H3 cells sensitized with DNP-IgE/HSA. Arrows in B indicate that the cells morphology became irregular, and purple granules were released outside of the cells.
Figure 5
Figure 5
Effects of coptisine on Alexa Fluor-488 phalloidin staining in DNP-IgE/HSA-sensitized cells. (A) Normal RBL-2H3 cells; (B) DNP-IgE/HSA-sensitized RBL-2H3 cells; (C) ketotifen fumarate-pretreated RBL-2H3 cells sensitized with DNP-IgE/HSA; (D) coptisine (30 μM)-pretreated RBL-2H3 cells sensitized with DNP-IgE/HSA. Arrows in B indicate that the cells morphology became irregular due to disassembly of the F-actin cytoskeleton.
Figure 6
Figure 6
Effect of coptisine on PI3K/Akt signaling in RBL-2H3 cells. The protein level and relative expression of (A) PI3K, (B) Akt were determined with Western blot. All data were expressed as the mean ± SE. *p < 0.05, **p < 0.01, in comparison with DNP-IgE/HSA group; ###p < 0.001 in comparison with control group.
Figure 7
Figure 7
Effect of coptisine on the number of nasal rubbing in OVA induced AR mice. Counting the number of nasal rubbing in the ten min after OVA intranasal injection. All data were expressed as the mean ± SE. ###p < 0.001, ##p < 0.01 were compared to the control group; *p < 0.05, **p < 0.01 and ***p < 0.001 were compared to AR mice, n = 10/group.
Figure 8
Figure 8
Effect of coptisine on OVA-specific IgE, histamine levels in OVA induced AR mice. The level of OVA-specific IgE (A); The level of histamine (B) All data were expressed as the mean ± SE. ##p < 0.01 were compared to the normal group; *p < 0.05 and **p < 0.01 were compared to AR mice, n = 10/group.
Figure 9
Figure 9
Effect of coptisine on IL-4 and TNF-α levels in OVA induced AR mice. Mice were intraperitoneally injected with OVA (50 μg) in aluminum hydroxide (2 mg) once every two days at day 0–14. Then, mice were sensitized by instilling 10 μL of 10% OVA into the bilateral nasal cavities for ten consecutive day. Mice received coptisine or ketotifen fumarate before the intranasal OVA challenge for ten day. The levels of (A) IL-4, and (B) TNF-α were measured by the ELISA method. All data were expressed as the mean ± SE. ##p < 0.01 were compared to the control group; *p < 0.05, **p < 0.01 were compared to AR mice, n = 10/group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Matsuoka D., Mizutani N., Sae-Wong C., Yoshino S. Allergen-specific regulation of allergic rhinitis in mice by intranasal exposure to IgG1 monoclonal antibody Fab fragments against pathogenic allergen. Immunol. Lett. 2014;161:149–156. doi: 10.1016/j.imlet.2014.06.005. - DOI - PubMed
    1. Nam S.Y., Chung C.K., Seo J.H., Rah S.Y., Kim H.M., Jeong H.J. The therapeutic efficacy of α-pinene in an experimental mouse model of allergic rhinitis. Int. Immunopharmacol. 2014;23:273–282. doi: 10.1016/j.intimp.2014.09.010. - DOI - PubMed
    1. Won J.H., Jung J.K., Weon C.C., Kang J.S., Park Y.K. Antiallergic effect of KOB03, a polyherbal medicine, on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse and human mast cells. J. Ethnopharmacol. 2012;142:684–693. - PubMed
    1. Fu M., Fu S., Ni S., Zou L., Liu Y., Hong T. Anti-inflammatory effect of epigallocatechin gallate in a mouse model of ovalbumin-induced allergic rhinitis. Int. Immunopharmacol. 2017;49:102–108. doi: 10.1016/j.intimp.2017.05.030. - DOI - PubMed
    1. Wu T.F., Chan Y.Y., Shi W.Y., Jhong M.T. Uncovering the Molecular Mechanism of Anti-Allergic Activity of Silkworm Pupa-Grown Cordyceps militaris Fruit Body. Am. J. Chin. Med. 2017;45:497–513. doi: 10.1142/S0192415X17500306. - DOI - PubMed

MeSH terms

Substances

Related information

LinkOut - more resources

Full text links
MDPI full text link MDPI Free PMC article
Cite
Send To

NCBI Literature Resources

MeSHPMCBookshelfDisclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

[8]ページ先頭
©2009-2025 Movatter.jp