Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family
- PMID:2948189
- PMCID: PMC304212
- DOI: 10.1073/pnas.84.2.388
Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family
Abstract
Mixed oligonucleotide probes were used to screen a HeLa cDNA library for clones encoding amino acid contiguities whose conservation is characteristic of the protein-serine kinase family. Eighty thousand clones were screened, from which 19 were identified as showing strong hybridization to two distinct probes. Four clones were chosen for characterization by partial DNA sequence analysis and 3 of these were found to encode amino acid sequences typical of protein-serine kinases. One deduced amino acid sequence shares 72% identity with rabbit skeletal muscle phosphorylase kinase gamma-subunit, while another is closely related to the yeast protein-serine kinases CDC2 in Schizosaccharomyces pombe and CDC28 in Saccharomyces cerevisiae. This screening approach should have applications in the identification of clones encoding previously unknown or poorly characterized members of other protein families.
Similar articles
- Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.Richardson HE, Stueland CS, Thomas J, Russell P, Reed SI.Richardson HE, et al.Genes Dev. 1990 Aug;4(8):1332-44. doi: 10.1101/gad.4.8.1332.Genes Dev. 1990.PMID:2227411
- Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2+ and suc1+.Draetta G, Brizuela L, Potashkin J, Beach D.Draetta G, et al.Cell. 1987 Jul 17;50(2):319-25. doi: 10.1016/0092-8674(87)90227-3.Cell. 1987.PMID:3297353
- cDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein.Cimprich KA, Shin TB, Keith CT, Schreiber SL.Cimprich KA, et al.Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2850-5. doi: 10.1073/pnas.93.7.2850.Proc Natl Acad Sci U S A. 1996.PMID:8610130Free PMC article.
- The role of workhorse protein kinases in coordinating DNA metabolism and cell growth.Christenson E, DeMaggio AJ, Hockstra MF.Christenson E, et al.Recent Results Cancer Res. 1997;143:263-74. doi: 10.1007/978-3-642-60393-8_19.Recent Results Cancer Res. 1997.PMID:8912426Review.No abstract available.
- Conservation and reiteration of a kinase cascade.Neiman AM.Neiman AM.Trends Genet. 1993 Nov;9(11):390-4. doi: 10.1016/0168-9525(93)90139-9.Trends Genet. 1993.PMID:8310536Review.
Cited by
- Identification of new protein kinase-related genes in three herpesviruses, herpes simplex virus, varicella-zoster virus, and Epstein-Barr virus.Smith RF, Smith TF.Smith RF, et al.J Virol. 1989 Jan;63(1):450-5. doi: 10.1128/JVI.63.1.450-455.1989.J Virol. 1989.PMID:2535748Free PMC article.
- p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes.Shuttleworth J, Godfrey R, Colman A.Shuttleworth J, et al.EMBO J. 1990 Oct;9(10):3233-40. doi: 10.1002/j.1460-2075.1990.tb07522.x.EMBO J. 1990.PMID:2209545Free PMC article.
- Molecular cloning of a second form of rac protein kinase.Jones PF, Jakubowicz T, Hemmings BA.Jones PF, et al.Cell Regul. 1991 Dec;2(12):1001-9. doi: 10.1091/mbc.2.12.1001.Cell Regul. 1991.PMID:1801921Free PMC article.
- Non-canonical approaches to targeting hypoxic tumors.Zhao S, El-Deiry WS.Zhao S, et al.Am J Cancer Res. 2022 Dec 15;12(12):5351-5374. eCollection 2022.Am J Cancer Res. 2022.PMID:36628275Free PMC article.Review.
- Interferon modulates the messenger RNA of G1-controlling genes to suppress the G1-to-S transition in Daudi cells.Yamada H, Ochi K, Nakada S, Takahara S, Nemoto T, Sekikawa T, Horiguchi-Yamada J.Yamada H, et al.Mol Cell Biochem. 1995 Nov 22;152(2):149-58. doi: 10.1007/BF01076077.Mol Cell Biochem. 1995.PMID:8751161
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Miscellaneous