Multilocus Sequence Typing of the Clinical Isolates ofSalmonella Enterica Serovar Typhimurium in Tehran Hospitals
- PMID:29234176
- PMCID: PMC5722961
Multilocus Sequence Typing of the Clinical Isolates ofSalmonella Enterica Serovar Typhimurium in Tehran Hospitals
Abstract
Background:Salmonella enterica serovar Typhimurium is one of the most important serovars ofSalmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics ofSalmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics ofSalmonella Typhimurium using multilocus sequence typing (MLST).
Methods: Clinical samples (urine, blood, and stool) were collected from patients, who were admitted to 2 hospitals in Tehran between April and September, 2015.Salmonella Typhimurium strains were identified by conventional standard biochemical and serological testing. The antibiotic susceptibility patterns of theSalmonella Typhimurium isolates against 16 antibiotics was determined using the disk diffusion assay. The clonal relationship between the strains ofSalmonella Typhimurium was analyzed using MLST.
Results: Among the 68Salmonella isolates, 31% (n=21) wereSalmonella Typhimurium. Of the total 21Salmonella Typhimurium isolates, 76% (n=16) were multidrug-resistant and showed resistance to 3 or more antibiotic families. TheSalmonella Typhimurium isolates were assigned to 2 sequence types: ST19 and ST328. ST19 was more common (86%). Both sequence types were further assigned to 1 eBURST group.
Conclusion: This is the first study of its kind in Iran to determine the sequence types of the clinical isolates ofSalmonella Typhimurium in Tehran hospitals using MLST. ST19 was detected as the major sequence type ofSalmonella Typhimurium.
Keywords: Iran; Multilocus sequence typing; Salmonella Typhimurium.
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