Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov
- PMID:29058645
- DOI: 10.1099/ijsem.0.002350
Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov
Abstract
Two rapidly growing mycobacteria with identical 16S rRNA gene sequences were the subject of a polyphasic taxonomic study. The strains formed a well-supported subclade in the mycobacterial 16S rRNA gene tree and were most closely associated with the type strain of Mycobacterium novocastrense. Single and multilocus sequence analyses based on hsp65, rpoB and 16S rRNA gene sequences showed that strains SN 1900T and SN 1904T are phylogenetically distinct but share several chemotaxonomic and phenotypic features that are are consistent with their classification in the genus Mycobacterium. The two strains were distinguished by their different fatty acid and mycolic acid profiles, and by a combination of phenotypic features. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values for strains SN 1900T and SN 1904T were 61.0 % and 94.7 %, respectively; in turn, the corresponding dDDH and ANI values with M. novocastrense DSM 44203T were 41.4 % and 42.8 % and 89.3 % and 89.5 %, respectively. These results show that strains SN1900T and SN 1904T form new centres of taxonomic variation within the genus Mycobacterium. Consequently, strains SN 1900T (40T=CECT 8763T=DSM 43219T) and SN 1904T (2409T=CECT 8766T=DSM 43532T) are considered to represent novel species, for which the names Mycobacteriumlehmannii sp. nov. and Mycobacteriumneumannii sp. nov. are proposed. A strain designated as 'Mycobacteriumacapulsensis' was shown to be a bona fide member of the putative novel species, M. lehmannii.
Keywords: Actinobacteria; draft-genome sequencing; polyphasic taxonomy.
Similar articles
- Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., two rapidly growing members of the genus Mycobacterium.Zhang DF, Chen X, Zhang XM, Zhi XY, Yao JC, Jiang Y, Xiong Z, Li WJ.Zhang DF, et al.Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4081-4086. doi: 10.1099/ijs.0.050567-0. Epub 2013 May 31.Int J Syst Evol Microbiol. 2013.PMID:23728378
- Formal description of Mycobacterium neglectum sp. nov. and Mycobacterium palauense sp. nov., rapidly growing actinobacteria.Nouioui I, Carro L, Sangal V, Jando M, Igual JM, Goodfellow M, Klenk HP.Nouioui I, et al.Antonie Van Leeuwenhoek. 2018 Jul;111(7):1209-1223. doi: 10.1007/s10482-018-1029-5. Epub 2018 Feb 5.Antonie Van Leeuwenhoek. 2018.PMID:29404824
- Mycobacterium eburneum sp. nov., a non-chromogenic, fast-growing strain isolated from sputum.Nouioui I, Carro L, Teramoto K, Igual JM, Jando M, Del Carmen Montero-Calasanz M, Sutcliffe I, Sangal V, Goodfellow M, Klenk HP.Nouioui I, et al.Int J Syst Evol Microbiol. 2017 Sep;67(9):3174-3181. doi: 10.1099/ijsem.0.002033. Epub 2017 Sep 4.Int J Syst Evol Microbiol. 2017.PMID:28869002
- A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species.Ramasamy D, Mishra AK, Lagier JC, Padhmanabhan R, Rossi M, Sentausa E, Raoult D, Fournier PE.Ramasamy D, et al.Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):384-391. doi: 10.1099/ijs.0.057091-0.Int J Syst Evol Microbiol. 2014.PMID:24505076Review.
- Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s.Tortoli E.Tortoli E.Clin Microbiol Rev. 2003 Apr;16(2):319-54. doi: 10.1128/CMR.16.2.319-354.2003.Clin Microbiol Rev. 2003.PMID:12692101Free PMC article.Review.
Cited by
- Species Diversity, Molecular Characterization, and Antimicrobial Susceptibility of Opportunistic Actinomycetes Isolated from Immunocompromised and Healthy Patients of Markazi Province of Iran.Azadi D, Motallebirad T, Ghaffari K, Shokri D, Rezaei F.Azadi D, et al.Infect Drug Resist. 2020 Jan 7;13:1-10. doi: 10.2147/IDR.S234292. eCollection 2020.Infect Drug Resist. 2020.PMID:32021315Free PMC article.
- An Update on Mycobacterial Taxonomy, 2016-2017.Parrish N.Parrish N.J Clin Microbiol. 2019 Apr 26;57(5):e01408-18. doi: 10.1128/JCM.01408-18. Print 2019 May.J Clin Microbiol. 2019.PMID:30602442Free PMC article.Review.
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials