doi: 10.1073/pnas.74.2.560.
A new method for sequencing DNA
- PMID:265521
- PMCID: PMC392330
- DOI: 10.1073/pnas.74.2.560
Item in Clipboard
A new method for sequencing DNA
A M Maxam et al. Proc Natl Acad Sci U S A.1977 Feb.
Display options
Format
Display options
Format
Abstract
DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.
Similar articles
- Mapping adenines, guanines, and pyrimidines in RNA.Donis-Keller H, Maxam AM, Gilbert W.Donis-Keller H, et al.Nucleic Acids Res. 1977 Aug;4(8):2527-38. doi: 10.1093/nar/4.8.2527.Nucleic Acids Res. 1977.PMID:409999Free PMC article.
- A new technique of pH gradient electrophoresis as applied to the separation of nucleic acid bases.Narurkar MV, Narurkar LM, Sahasrabudhe MB.Narurkar MV, et al.Anal Biochem. 1968 Oct 10;26(1):174-7. doi: 10.1016/0003-2697(68)90040-7.Anal Biochem. 1968.PMID:5758026No abstract available.
- Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length.Carroll D, Brown DD.Carroll D, et al.Cell. 1976 Apr;7(4):467-75. doi: 10.1016/0092-8674(76)90198-7.Cell. 1976.PMID:986247
- [Gas chromatography of nucleic acid components].Hashizume T, Sasaki Y.Hashizume T, et al.Tanpakushitsu Kakusan Koso. 1968 Jul;13(8):735-42.Tanpakushitsu Kakusan Koso. 1968.PMID:4882393Review.Japanese.No abstract available.
- Sequencing 5-methylcytosine residues in genomic DNA.Grigg G, Clark S.Grigg G, et al.Bioessays. 1994 Jun;16(6):431-6. doi: 10.1002/bies.950160612.Bioessays. 1994.PMID:8080433Review.
Cited by
- My Early Years of Yeast Mitochondrial Genetics.Macreadie IG.Macreadie IG.Microorganisms. 2024 Oct 17;12(10):2077. doi: 10.3390/microorganisms12102077.Microorganisms. 2024.PMID:39458386Free PMC article.Review.
- Recent Advances in Autism Spectrum Disorders: Applications of Whole Exome Sequencing Technology.Sener EF, Canatan H, Ozkul Y.Sener EF, et al.Psychiatry Investig. 2016 May;13(3):255-64. doi: 10.4306/pi.2016.13.3.255. Epub 2016 May 18.Psychiatry Investig. 2016.PMID:27247591Free PMC article.Review.
- Connecting environment and genome plasticity in the characterization of transformation-induced SOS regulation and carbon catabolite control of the Vibrio cholerae integron integrase.Baharoglu Z, Krin E, Mazel D.Baharoglu Z, et al.J Bacteriol. 2012 Apr;194(7):1659-67. doi: 10.1128/JB.05982-11. Epub 2012 Jan 27.J Bacteriol. 2012.PMID:22287520Free PMC article.
- Molecular Analyses in Peritoneal Metastasis from Colorectal Cancer: A Review-An English Version.Kim CH.Kim CH.J Anus Rectum Colon. 2022 Oct 27;6(4):197-202. doi: 10.23922/jarc.2022-045. eCollection 2022.J Anus Rectum Colon. 2022.PMID:36348949Free PMC article.Review.
- The transcription factor LF-A1 interacts with a bipartite recognition sequence in the promoter regions of several liver-specific genes.Ramji DP, Tadros MH, Hardon EM, Cortese R.Ramji DP, et al.Nucleic Acids Res. 1991 Mar 11;19(5):1139-46. doi: 10.1093/nar/19.5.1139.Nucleic Acids Res. 1991.PMID:2020548Free PMC article.
References
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources