Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
- PMID:26477261
- DOI: 10.1016/bs.mie.2015.06.040
Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
Abstract
Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (<1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
Keywords: Hydration dynamics; Overhauser dynamic nuclear polarization; Protein hydration water measurements; Site-directed hydration dynamics; Site-directed spin labeling.
© 2015 Elsevier Inc. All rights reserved.
Similar articles
- Overhauser Dynamic Nuclear Polarization for the Study of Hydration Dynamics, Explained.Franck JM, Han S.Franck JM, et al.Methods Enzymol. 2019;615:131-175. doi: 10.1016/bs.mie.2018.09.024. Epub 2018 Dec 11.Methods Enzymol. 2019.PMID:30638529Review.
- Dynamic nuclear polarization enhanced nuclear magnetic resonance and electron spin resonance studies of hydration and local water dynamics in micelle and vesicle assemblies.McCarney ER, Armstrong BD, Kausik R, Han S.McCarney ER, et al.Langmuir. 2008 Sep 16;24(18):10062-72. doi: 10.1021/la800334k. Epub 2008 Aug 14.Langmuir. 2008.PMID:18700788
- Cholesterol enhances surface water diffusion of phospholipid bilayers.Cheng CY, Olijve LL, Kausik R, Han S.Cheng CY, et al.J Chem Phys. 2014 Dec 14;141(22):22D513. doi: 10.1063/1.4897539.J Chem Phys. 2014.PMID:25494784Free PMC article.
- An ultrasensitive tool exploiting hydration dynamics to decipher weak lipid membrane-polymer interactions.Cheng CY, Wang JY, Kausik R, Lee KY, Han S.Cheng CY, et al.J Magn Reson. 2012 Feb;215:115-9. doi: 10.1016/j.jmr.2011.12.004. Epub 2011 Dec 10.J Magn Reson. 2012.PMID:22230738
- Quantitative cw Overhauser effect dynamic nuclear polarization for the analysis of local water dynamics.Franck JM, Pavlova A, Scott JA, Han S.Franck JM, et al.Prog Nucl Magn Reson Spectrosc. 2013 Oct;74:33-56. doi: 10.1016/j.pnmrs.2013.06.001. Epub 2013 Jul 4.Prog Nucl Magn Reson Spectrosc. 2013.PMID:24083461Free PMC article.Review.
Cited by
- Spatially Heterogeneous Surface Water Diffusivity around Structured Protein Surfaces at Equilibrium.Barnes R, Sun S, Fichou Y, Dahlquist FW, Heyden M, Han S.Barnes R, et al.J Am Chem Soc. 2017 Dec 13;139(49):17890-17901. doi: 10.1021/jacs.7b08606. Epub 2017 Nov 27.J Am Chem Soc. 2017.PMID:29091442Free PMC article.
- Functional Role of Solvent Entropy and Conformational Entropy of Metal Binding in a Dynamically Driven Allosteric System.Capdevila DA, Edmonds KA, Campanello GC, Wu H, Gonzalez-Gutierrez G, Giedroc DP.Capdevila DA, et al.J Am Chem Soc. 2018 Jul 25;140(29):9108-9119. doi: 10.1021/jacs.8b02129. Epub 2018 Jul 16.J Am Chem Soc. 2018.PMID:29953213Free PMC article.
- Spin-labeling Insights into How Chemical Fixation Impacts Glycan Organization on Cells.Jaiswal M, Tran TT, Guo J, Zhou M, Kundu S, Guo Z, Fanucci GE.Jaiswal M, et al.Appl Magn Reson. 2024 Mar;55(1-3):317-333. doi: 10.1007/s00723-023-01624-w. Epub 2023 Oct 9.Appl Magn Reson. 2024.PMID:38469359Free PMC article.
- gem-Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility.Bleicken S, Assafa TE, Zhang H, Elsner C, Ritsch I, Pink M, Rajca S, Jeschke G, Rajca A, Bordignon E.Bleicken S, et al.ChemistryOpen. 2019 May 14;8(8):1057-1065. doi: 10.1002/open.201900119. eCollection 2019 Aug.ChemistryOpen. 2019.PMID:31463171Free PMC article.
- Structure of Membrane-Bound Huntingtin Exon 1 Reveals Membrane Interaction and Aggregation Mechanisms.Tao M, Pandey NK, Barnes R, Han S, Langen R.Tao M, et al.Structure. 2019 Oct 1;27(10):1570-1580.e4. doi: 10.1016/j.str.2019.08.003. Epub 2019 Aug 26.Structure. 2019.PMID:31466833Free PMC article.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources