.2015 Sep 28;34(6):632-41.

doi: 10.1016/j.devcel.2015.08.018.

Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

Jin Xu¹, Lu Zhu¹, Sicong He², Yi Wu¹, Wan Jin¹, Tao Yu¹, Jianan Y Qu³, Zilong Wen⁴

Affiliations

¹ Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
² Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
³ Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: eequ@ust.hk.
⁴ Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: zilong@ust.hk.

PMID:26418294
DOI: 10.1016/j.devcel.2015.08.018

Free article

Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

Jin Xu et al. Dev Cell.2015.

Free article

.2015 Sep 28;34(6):632-41.

doi: 10.1016/j.devcel.2015.08.018.

Authors

Jin Xu¹, Lu Zhu¹, Sicong He², Yi Wu¹, Wan Jin¹, Tao Yu¹, Jianan Y Qu³, Zilong Wen⁴

Affiliations

¹ Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
² Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
³ Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: eequ@ust.hk.
⁴ Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: zilong@ust.hk.

PMID:26418294
DOI: 10.1016/j.devcel.2015.08.018

Abstract

Microglia are CNS resident macrophages, and they play important roles in neural development and function. Recent studies have suggested that murine microglia arise from a single source, the yolk sac (YS), yet these studies lack spatial resolution to define the bona fide source(s) for microglia. Here, using light-induced high temporal-spatial resolution fate mapping, we challenge this single-source view by showing that microglia in zebrafish arise from multiple sources. The embryonic/larval microglia originate from the rostral blood island (RBI) region, the equivalent of mouse YS for myelopoiesis, whereas the adult microglia arise from the ventral wall of dorsal aorta (VDA) region, a tissue also producing definitive hematopoiesis in mouse. We further show that the VDA-region-derived microglia are Runx1 dependent, but cMyb independent, and developmentally regulated differently from the RBI region-derived microglia. Our study establishes a new paradigm for investigating the development and function of distinct microglia populations.

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Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

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Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

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