.2015 Mar 3:5:8706.

doi: 10.1038/srep08706.

A laboratory investigation of interactions between denitrifying anaerobic methane oxidation (DAMO) and anammox processes in anoxic environments

Shihu Hu¹, Raymond J Zeng², Mohamed F Haroon³, Jurg Keller¹, Paul A Lant⁴, Gene W Tyson³, Zhiguo Yuan¹

Affiliations

¹ Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia.
² 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] Department of Chemistry, University of Science &Technology of China, Hefei. 230026, China.
³ 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] Australian Centre for Ecogenomics, The University of Queensland, Brisbane. 4072, Australia.
⁴ 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] School of Chemical Engineering, The University of Queensland, Brisbane. 4072, Australia.

PMID:25732131
PMCID: PMC4346804
DOI: 10.1038/srep08706

A laboratory investigation of interactions between denitrifying anaerobic methane oxidation (DAMO) and anammox processes in anoxic environments

Shihu Hu et al. Sci Rep.2015.

.2015 Mar 3:5:8706.

doi: 10.1038/srep08706.

Authors

Shihu Hu¹, Raymond J Zeng², Mohamed F Haroon³, Jurg Keller¹, Paul A Lant⁴, Gene W Tyson³, Zhiguo Yuan¹

Affiliations

¹ Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia.
² 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] Department of Chemistry, University of Science &Technology of China, Hefei. 230026, China.
³ 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] Australian Centre for Ecogenomics, The University of Queensland, Brisbane. 4072, Australia.
⁴ 1] Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane. 4072, Australia [2] School of Chemical Engineering, The University of Queensland, Brisbane. 4072, Australia.

PMID:25732131
PMCID: PMC4346804
DOI: 10.1038/srep08706

Abstract

This study investigates interactions between recently identified denitrifying anaerobic methane oxidation (DAMO) and anaerobic ammonium oxidation (anammox) processes in controlled anoxic laboratory reactors. Two reactors were seeded with the same inocula containing DAMO organisms Candidatus Methanoperedens nitroreducens and Candidatus Methylomirabilis oxyfera, and anammox organism Candidatus Kuenenia stuttgartiensis. Both were fed with ammonium and methane, but one was also fed with nitrate and the other with nitrite, providing anoxic environments with different electron acceptors. After steady state reached in several months, the DAMO process became solely/primarily responsible for nitrate reduction while the anammox process became solely responsible for nitrite reduction in both reactors. 16S rRNA gene amplicon sequencing showed that the nitrate-driven DAMO organism M. nitroreducens dominated both the nitrate-fed (~70%) and the nitrite-fed (~26%) reactors, while the nitrite-driven DAMO organism M. oxyfera disappeared in both communities. The elimination of M. oxyfera from both reactors was likely the results of this organism being outcompeted by anammox bacteria for nitrite. K. stuttgartiensis was detected at relatively low levels (1-3%) in both reactors.

PubMed Disclaimer

Figures

**Figure 1. Nitrogen and carbon transformation in the nitrate-fed reactor over the 360 days of reactor operation.**
(a): temporal profiles of methane () and nitrogen gas (), which represent the total amount of these substances in both the gas and liquid phases; (b): temporal profiles of ammonium (), nitrate () and nitrite (). The concentration of nitrite () was close to zero most of time; (c): The NO₂⁻ reduction rate by anammox (, r_anammox), NO₂⁻ reduction rate by DAMO (, r_DAMO-nitrite) and NO₃⁻ reduction rate by DAMO (, r_DAMO-nitrate) calculated based on liquid phase measurements and reaction stoichiometry.

formula image — **Figure 1. Nitrogen and carbon transformation in the nitrate-fed reactor over the 360 days of reactor operation.**
(a): temporal profiles of methane () and nitrogen gas (), which represent the total amount of these substances in both the gas and liquid phases; (b): temporal profiles of ammonium (), nitrate () and nitrite (). The concentration of nitrite () was close to zero most of time; (c): The NO₂⁻ reduction rate by anammox (, r_anammox), NO₂⁻ reduction rate by DAMO (, r_DAMO-nitrite) and NO₃⁻ reduction rate by DAMO (, r_DAMO-nitrate) calculated based on liquid phase measurements and reaction stoichiometry.

**Figure 2. Community dynamics in the nitrate-fed reactor.**
FISH images of sludge samples fixed on Days 0 (a) and Day 259 (b) after hybridization with specific probes: Cy3 ARC872 forM. nitroreducens (red), Cy5 NC1162 forM. oxyfera (blue) and FITC AMX820 for anammox bacteria (green). (c) Microbial community composition determined by sequencing in the bioreactor on Day 259.

**Figure 3. DAMO and anammox activities in nitrite-fed reactor.**
(a) and (b): The consumption of methane (), ammonium () and nitrate (), and production of nitrogen gas () were observed. The loading rate of nitrite (−) was constant from Day 57 onwards except between Days 230 to 234, where nitrate and nitrite was added manually during batch tests (data not shown). (c): The NO₂⁻ reduction rate by anammox (, r_anammox), NO₂⁻ reduction rate by DAMO (, r_DAMO-nitrite) and NO₃⁻ reduction rate by DAMO (, r_DAMO-nitrate) calculated based on liquid phase measurements and reaction stoichiometry.

**Figure 4. Variations of populations of anammox and DAMO microorganisms in the nitrite-fed reactor.**
FISH images of sludge samples fixed on Days 0 (a) and 259 (b) after hybridization with specific probes: Cy3 ARC872 forM. nitroreducens (red), Cy5 NC1162 forM. oxyfera (blue) and FITC AMX820 for anammox bacteria (green). (c): Microbial community composition determined by sequencing in the nitrite-fed reactor on Day 259.

**Figure 5. A conceptual model for the interactions between the DAMO and anammox processes in an anoxic environment rich in methane, ammonium and nitrate or nitrite.**
Material flow and electron flow are shown in solid and dashed lines, respectively.

See this image and copyright information in PMC

Cited by

Evolution of the microbial community of the biofilm in a methane-based membrane biofilm reactor reducing multiple electron acceptors.
Chen R, Luo YH, Chen JX, Zhang Y, Wen LL, Shi LD, Tang Y, Rittmann BE, Zheng P, Zhao HP.Chen R, et al.Environ Sci Pollut Res Int. 2016 May;23(10):9540-8. doi: 10.1007/s11356-016-6146-y. Epub 2016 Feb 3.Environ Sci Pollut Res Int. 2016.PMID:26841777
Microbial Biogeochemical Cycling of Nitrogen in Arid Ecosystems.
Ramond JB, Jordaan K, Díez B, Heinzelmann SM, Cowan DA.Ramond JB, et al.Microbiol Mol Biol Rev. 2022 Jun 15;86(2):e0010921. doi: 10.1128/mmbr.00109-21. Epub 2022 Apr 7.Microbiol Mol Biol Rev. 2022.PMID:35389249Free PMC article.Review.
Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment.
Black EM, Chimenti MS, Just CL.Black EM, et al.PeerJ. 2017 Jul 12;5:e3536. doi: 10.7717/peerj.3536. eCollection 2017.PeerJ. 2017.PMID:28717594Free PMC article.
The Role of Land Use Types and Water Chemical Properties in Structuring the Microbiomes of a Connected Lake System.
Marmen S, Blank L, Al-Ashhab A, Malik A, Ganzert L, Lalzar M, Grossart HP, Sher D.Marmen S, et al.Front Microbiol. 2020 Feb 12;11:89. doi: 10.3389/fmicb.2020.00089. eCollection 2020.Front Microbiol. 2020.PMID:32117119Free PMC article.
Long-term nitrate removal through methane-dependent denitrification microorganisms in sequencing batch reactors fed with only nitrate and methane.
Li W, Lu P, Chai F, Zhang L, Han X, Zhang D.Li W, et al.AMB Express. 2018 Jun 30;8(1):108. doi: 10.1186/s13568-018-0637-9.AMB Express. 2018.PMID:29961200Free PMC article.

See all "Cited by" articles

References

1. Kuenen J. G. Anammox bacteria: from discovery to application. Nat. Rev. Microbiol. 6, 320–326 (2008). - PubMed
1. Kuypers M. M. M. et al. Anaerobic ammonium oxidation by anammox bacteria in the Black Sea. Nature 422, 608–611 (2003). - PubMed
1. Dalsgaard T., Canfield D. E., Petersen J., Thamdrup B. & Acuna-Gonzalez J. N2 production by the anammox reaction in the anoxic water column of Golfo Dulce, Costa Rica. Nature 422, 606–608 (2003). - PubMed
1. Jetten M. S. M. et al. Biochemistry and molecular biology of anammox bacteria. Crit. Rev. Biochem. Mol. Biol. 44, 65–84 (2009). - PubMed
1. Strous M. & Jetten M. S. M. Anaerobic oxidation of methane and ammonium. Annu. Rev. Microbiol. 58, 99–117 (2004). - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions

Related information

PubChem Compound (MeSH Keyword)

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

Movatterモバイル変換

Account

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Full text links

Actions

Share

A laboratory investigation of interactions between denitrifying anaerobic methane oxidation (DAMO) and anammox processes in anoxic environments

Affiliations

A laboratory investigation of interactions between denitrifying anaerobic methane oxidation (DAMO) and anammox processes in anoxic environments

Authors

Affiliations

Abstract

Figures

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Other Literature Sources