doi: 10.1016/j.mimet.2014.10.016.
Improvement in the DNA sequencing of genomes bearing long repeated elements
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- PMID:25447886
- DOI: 10.1016/j.mimet.2014.10.016
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Improvement in the DNA sequencing of genomes bearing long repeated elements
Antony T Vincent et al. J Microbiol Methods.2014 Dec.
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Abstract
When using next-generation sequencing methods, obtaining a single and complete bacterial chromosomic sequence may be challenging especially for bacteria harboring multiple repeated sequences. Here we present a one-step procedure to optimize the sequencing process in order to obtain a single chromosomic scaffold.
Copyright © 2014 Elsevier B.V. All rights reserved.
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