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.2013 Oct 2:30:9D.2.1-9D.2.13.
doi: 10.1002/9780471729259.mc09d02s30.

Laboratory growth and maintenance of Streptococcus pyogenes (the Group A Streptococcus, GAS)

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Laboratory growth and maintenance of Streptococcus pyogenes (the Group A Streptococcus, GAS)

Kanika Gera et al. Curr Protoc Microbiol..

Abstract

Streptococcus pyogenes is a Gram-positive bacterium that strictly infects humans. It is the causative agent of a broad spectrum of diseases accounting for millions of infections and at least 517,000 deaths each year worldwide. It is a nutritionally fastidious organism that ferments sugars to produce lactic acid and has strict requirements for growth. To aid in the study of this organism, this unit describes the growth and maintenance of S. pyogenes.

Keywords: Streptococcus pyogenes; chemically defined medium; facultative anaerobe; firmicute; the Group A Streptococcus.

Copyright © 2013 John Wiley & Sons, Inc.

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Figures

Figure 1
Figure 1
Types of hemolysis observed upon growth on sheep blood agar: Alpha (α) hemolysis (top) exhibits incomplete clearance with a zone of greenish discoloration around the colony (e.g.Streptococcus pneumoniae). Gamma (γ) hemolysis (center) shows no clearing around the colony (e.g.Enterococcus faecalis). Beta (β) hemolysis (bottom) produces a clear zone of hemolysis immediately around the colony (e.g.Streptococcus pyogenes). Image published with permission of Lippencott, Williams, and Wilkins Press (http://lww.com).
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