doi: 10.1039/c3lc50945a.
Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics
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- PMID:24185478
- DOI: 10.1039/c3lc50945a
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Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics
Venkatachalam Chokkalingam et al. Lab Chip..
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Abstract
Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.
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