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Comparative Study
.1990 Oct;71(4):792-6.
doi: 10.1210/jcem-71-4-792.

Deoxyribonucleic acid analyses of five families with familial inherited thyroid stimulating hormone deficiency

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Comparative Study

Deoxyribonucleic acid analyses of five families with familial inherited thyroid stimulating hormone deficiency

Y Hayashizaki et al. J Clin Endocrinol Metab.1990 Oct.

Abstract

Five families with familial inherited TSH deficiency, reported to date, were examined for the TSH beta gene at the nucleotide level. The first family carries a single base substitution in the 29th codon which lies in the so-called CAGYC region; GCA (glycine) is replaced by AGA (arginine). This substitution induces conformational changes of the beta-polypeptide which make it unable to associate with the alpha-subunit. This mutation generates a new cleavage site for a restriction endonuclease MaeI, a new marker that can be used for DNA diagnosis. The second and third families were found to carry the same nucleotide substitution. Also, all three families were associated with an additional single base substitution in intron 2 as a polymorphic change, suggesting that these three families may have originated from the same single founder from Shikoku Island in Japan. The nucleotide sequence from the fourth and fifth families showed no alterations in the TSH beta gene from the about -200 basepair up-stream region to the polyadenylation site.

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Comment in

  • Genes and endocrine maladies.
    Chin WW.Chin WW.J Clin Endocrinol Metab. 1990 Oct;71(4):790-1. doi: 10.1210/jcem-71-4-790.J Clin Endocrinol Metab. 1990.PMID:2401710No abstract available.

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