Pathogenesis of Campylobacter fetus infections. Role of surface array proteins in virulence in a mouse model
- PMID:2318963
- PMCID: PMC296532
- DOI: 10.1172/JCI114533
Pathogenesis of Campylobacter fetus infections. Role of surface array proteins in virulence in a mouse model
Abstract
We developed a mouse model to compare the virulence of Campylobacter fetus strains with (S-plus) and without (S-minus) surface array protein (S-protein) capsules. In adult HA/ICR mice pretreated with ferric chloride, the LD50 for S-plus strain 84-32 was 43.3 times lower than its spontaneous S-minus mutant 84-54. Seven strains of inbred mice were no more susceptible than the outbred strain. In contrast to the findings with Salmonella typhimurium by others, 3 X 10(7) CFU of strain 84-32 caused 90% mortality in C3H/HeN (LPSn) mice and 40% mortality in C3H/HeJ (LPSd) mice. High-grade bacteremia in HA/ICR mice occurred after oral challenge with S-plus C. fetus strains and continued for at least 2 d, but was not present in any mice challenged with S-minus strains. Bacteremia at 30 min after challenge was 51.6-fold lower in mice pretreated with 10 microliters of rabbit antiserum to purified S-protein than after pretreatment with normal rabbit serum. Challenge of mice with a mixture of S-minus strain 84-54 and free S-proteins at a concentration 31.1-fold higher than found in wild-type strain 84-32 caused 30% mortality, compared with 0% with strain 84-54 or S-protein alone. These findings in a mouse model point toward the central role of the S-protein in the pathogenesis of C. fetus infection. The S-protein is not toxic per se, but enhances virulence when present on the bacterial cell surface as a capsule.
Similar articles
- Factors affecting the lethality of Campylobacter fetus subspecies jejuni in mice.Stewart-Tull DE, Ng FK, Wardlaw AC.Stewart-Tull DE, et al.J Med Microbiol. 1984 Aug;18(1):27-37. doi: 10.1099/00222615-18-1-27.J Med Microbiol. 1984.PMID:6748038
- Pathogenesis of Campylobacter fetus infections: critical role of high-molecular-weight S-layer proteins in virulence.Blaser MJ, Pei Z.Blaser MJ, et al.J Infect Dis. 1993 Feb;167(2):372-7. doi: 10.1093/infdis/167.2.372.J Infect Dis. 1993.PMID:8421171
- Relationship between immune system and gram-negative bacteria. IV. T lymphocytes from Lpsd mice possess binding site(s) for Rb Salmonella.Jirillo E, Antonaci S, Kiyono H, Michalek SM, McGhee JR.Jirillo E, et al.J Immunol. 1985 Nov;135(5):3473-8.J Immunol. 1985.PMID:3876386
- [Pathogenesis of Campylobacter fetus infection].Fujimoto S.Fujimoto S.Fukuoka Igaku Zasshi. 2003 Jul;94(7):225-34.Fukuoka Igaku Zasshi. 2003.PMID:14509230Review.Japanese.
- Role of the S-layer proteins of Campylobacter fetus in serum-resistance and antigenic variation: a model of bacterial pathogenesis.Blaser MJ.Blaser MJ.Am J Med Sci. 1993 Nov;306(5):325-9. doi: 10.1097/00000441-199311000-00011.Am J Med Sci. 1993.PMID:8238090Review.
Cited by
- So close and yet so far - Molecular Microbiology of Campylobacter fetus subspecies.Sprenger H, Zechner EL, Gorkiewicz G.Sprenger H, et al.Eur J Microbiol Immunol (Bp). 2012 Mar;2(1):66-75. doi: 10.1556/EuJMI.2.2012.1.10. Epub 2012 Mar 17.Eur J Microbiol Immunol (Bp). 2012.PMID:24611123Free PMC article.Review.
- Shift in S-layer protein expression responsible for antigenic variation in Campylobacter fetus.Wang E, Garcia MM, Blake MS, Pei Z, Blaser MJ.Wang E, et al.J Bacteriol. 1993 Aug;175(16):4979-84. doi: 10.1128/jb.175.16.4979-4984.1993.J Bacteriol. 1993.PMID:7688715Free PMC article.
- S-layer-mediated association of Aeromonas salmonicida with murine macrophages.Garduño RA, Lee EJ, Kay WW.Garduño RA, et al.Infect Immun. 1992 Oct;60(10):4373-82. doi: 10.1128/iai.60.10.4373-4382.1992.Infect Immun. 1992.PMID:1398947Free PMC article.
- Persistence of Campylobacter fetus bacteremia associated with absence of opsonizing antibodies.Neuzil KM, Wang E, Haas DW, Blaser MJ.Neuzil KM, et al.J Clin Microbiol. 1994 Jul;32(7):1718-20. doi: 10.1128/jcm.32.7.1718-1720.1994.J Clin Microbiol. 1994.PMID:7929763Free PMC article.
- Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids.Graaf-van Bloois Lv, Miller WG, Yee E, Gorkiewicz G, Forbes KJ, Zomer AL, Wagenaar JA, Duim B.Graaf-van Bloois Lv, et al.PLoS One. 2016 Apr 6;11(4):e0152832. doi: 10.1371/journal.pone.0152832. eCollection 2016.PLoS One. 2016.PMID:27049518Free PMC article.
References
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources