doi: 10.1128/aem.56.11.3368-3374.1990.
Luminescence-based nonextractive technique for in situ detection of Escherichia coli in soil
Affiliations
- PMID:2268151
- PMCID: PMC184955
- DOI: 10.1128/aem.56.11.3368-3374.1990
Item in Clipboard
Luminescence-based nonextractive technique for in situ detection of Escherichia coli in soil
E A Rattray et al. Appl Environ Microbiol.1990 Nov.
Display options
Format
Display options
Format
Abstract
Measurement of light output by luminometry was used to estimate quantitatively the cell concentrations of luminescent strains of Escherichia coli in liquid culture and inoculated into soil. Strains were constructed in which luciferase production was autoinducible or constitutive. In the former, light output per cell varied considerably during growth but was constant in constitutive strains. In liquid culture, the lower detection limit was in the order of 10(2) cells ml-1. Sensitivity was reduced by approximately 1 order of magnitude for cells inoculated into soil, when 2 x 10(2) to 6 x 10(3) cells g of soil-1 could be detected. Light output measurements were obtained within 5 min of sampling, and luminometry therefore potentially offers a rapid and sensitive detection technique for genetically engineered microorganisms.
Similar articles
- Luminometric measurement of population activity of genetically modified Pseudomonas fluorescens in the soil.Meikle A, Killham K, Prosser JI, Glover LA.Meikle A, et al.FEMS Microbiol Lett. 1992 Dec 1;78(2-3):217-20. doi: 10.1016/0378-1097(92)90029-n.FEMS Microbiol Lett. 1992.PMID:1490601
- Simultaneous monitoring of cell number and metabolic activity of specific bacterial populations with a dual gfp-luxAB marker system.Unge A, Tombolini R, Molbak L, Jansson JK.Unge A, et al.Appl Environ Microbiol. 1999 Feb;65(2):813-21. doi: 10.1128/AEM.65.2.813-821.1999.Appl Environ Microbiol. 1999.PMID:9925621Free PMC article.
- In vivo bioluminescence imaging for the study of intestinal colonization by Escherichia coli in mice.Foucault ML, Thomas L, Goussard S, Branchini BR, Grillot-Courvalin C.Foucault ML, et al.Appl Environ Microbiol. 2010 Jan;76(1):264-74. doi: 10.1128/AEM.01686-09. Epub 2009 Oct 30.Appl Environ Microbiol. 2010.PMID:19880653Free PMC article.
- Luminescence-based detection of activity of starved and viable but nonculturable bacteria.Duncan S, Glover LA, Killham K, Prosser JI.Duncan S, et al.Appl Environ Microbiol. 1994 Apr;60(4):1308-16. doi: 10.1128/aem.60.4.1308-1316.1994.Appl Environ Microbiol. 1994.PMID:8017919Free PMC article.
- Luminescence-based systems for detection of bacteria in the environment.Prosser JI, Killham K, Glover LA, Rattray EA.Prosser JI, et al.Crit Rev Biotechnol. 1996;16(2):157-83. doi: 10.3109/07388559609147420.Crit Rev Biotechnol. 1996.PMID:8635199Review.
Cited by
- Use of bioluminescence for detection of genetically engineered microorganisms released into the environment.Shaw JJ, Dane F, Geiger D, Kloepper JW.Shaw JJ, et al.Appl Environ Microbiol. 1992 Jan;58(1):267-73. doi: 10.1128/aem.58.1.267-273.1992.Appl Environ Microbiol. 1992.PMID:1311542Free PMC article.
- Effect of temperature, pH, and initial cell number on luxCDABE and nah gene expression during naphthalene and salicylate catabolism in the bioreporter organism Pseudomonas putida RB1353.Dorn JG, Frye RJ, Maier RM.Dorn JG, et al.Appl Environ Microbiol. 2003 Apr;69(4):2209-16. doi: 10.1128/AEM.69.4.2209-2216.2003.Appl Environ Microbiol. 2003.PMID:12676702Free PMC article.
- Bioluminescent Most-Probable-Number Method To Enumerate lux-Marked Pseudomonas aeruginosa UG2Lr in Soil.Flemming CA, Lee H, Trevors JT.Flemming CA, et al.Appl Environ Microbiol. 1994 Sep;60(9):3458-61. doi: 10.1128/aem.60.9.3458-3461.1994.Appl Environ Microbiol. 1994.PMID:16349396Free PMC article.
- Monitoring of Yersinia enterocolitica in murine and bovine feces on the basis of the chromosomally integrated luxAB marker gene.Kaniga K, Sory MP, Delor I, Saegerman C, Limet JN, Cornelis GR.Kaniga K, et al.Appl Environ Microbiol. 1992 Mar;58(3):1024-6. doi: 10.1128/aem.58.3.1024-1026.1992.Appl Environ Microbiol. 1992.PMID:1575474Free PMC article.
- Noninvasive quantitative measurement of bacterial growth in porous media under unsaturated-flow conditions.Yarwood RR, Rockhold ML, Niemet MR, Selker JS, Bottomley PJ.Yarwood RR, et al.Appl Environ Microbiol. 2002 Jul;68(7):3597-605. doi: 10.1128/AEM.68.7.3597-3605.2002.Appl Environ Microbiol. 2002.PMID:12089048Free PMC article.
References
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources