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Randomized Controlled Trial
.2012 May;19(5):642-8.
doi: 10.1128/CVI.00033-12. Epub 2012 Mar 29.

Immune responses and safety after dart or booster vaccination of bison with Brucella abortus strain RB51

Affiliations
Randomized Controlled Trial

Immune responses and safety after dart or booster vaccination of bison with Brucella abortus strain RB51

S C Olsen et al. Clin Vaccine Immunol.2012 May.

Abstract

One alternative for management of brucellosis in Yellowstone National Park bison (Bison bison) is vaccination of calves and yearlings. Although Brucella abortus strain RB51 vaccination protects bison against experimental challenge, the effect of booster vaccinations was unknown. This study characterized immunologic responses after dart or booster vaccination of bison with Brucella abortus strain RB51. In two studies, 8- to 10-month-old female bison were inoculated with saline (n = 14), hand vaccinated with 1.1 × 10(10) to 2.0 × 10(10) CFU of RB51 (n = 21), or dart vaccinated with 1.8 × 10(10) CFU of RB51 (n = 7). A subgroup of hand vaccinates in study 1 was randomly selected for booster vaccination 15 months later with 2.2 × 10(10) CFU of RB51. Compared to single vaccinates, booster-vaccinated bison had greater serologic responses to RB51. However, there was a trend for antigen-specific proliferative responses of peripheral blood mononuclear cells (PBMC) from booster vaccinates to be reduced compared to responses of PBMC from single vaccinates. PBMC from booster vaccinates tended to have greater gamma interferon (IFN-γ) production than those from single vaccinates. In general, dart vaccination with RB51 induced immunologic responses similar to those of hand vaccination. All vaccinates (single hand, dart, or booster) demonstrated greater (P < 0.05) immunologic responses at various times after vaccination than nonvaccinated bison. Booster vaccination with RB51 in early gestation did not induce abortion or fetal infection. Our data suggest that booster vaccination does not induce strong anamnestic responses. However, phenotypic data on resistance to experimental challenge are required to fully assess the effect of booster vaccination on protective immunity.

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Figures

Fig 1
Fig 1
Bison serologic responses to gamma-irradiated RB51 in an ELISA after initial inoculation with 1010 CFU RB51 and a booster vaccination 60 weeks later with a similar dose of RB51. Sera obtained after the initial and booster vaccinations were run in parallel on an ELISA plate. Responses are presented as the mean optical density ± the standard error of the mean (SEM). Means with different letters are significantly different (P < 0.05).
Fig 2
Fig 2
Serologic responses to gamma-irradiated RB51 in an ELISA of bison after hand inoculation (hand RB51) or pneumatic dart delivery (dart RB51) of 1010 CFU of RB51. Responses are presented as the mean optical density ± the SEM. Means with different letters are significantly different (P < 0.05).
Fig 3
Fig 3
Proliferative responses to 108 CFU of gamma-irradiated RB51 by peripheral blood mononuclear cells from bison vaccinated with saline or 1010 CFU of RB51. Cells were incubated at 37°C and 5% CO2 for 7 days and pulsed for 18 h with [3H]thymidine. Means within a sampling time with different letters are significantly different (P < 0.05). The mean response of control bison PBMC incubated in the absence of antigen was 5,374 ± 2,400.
Fig 4
Fig 4
Proliferative responses to 108 CFU of gamma-irradiated RB51 by peripheral blood mononuclear cells from bison initially vaccinated with saline (control), bison initially vaccinated with 1010 CFU of RB51 (hand RB51 once), or bison initially vaccinated and then booster vaccinated 60 weeks later with 1010 CFU of RB51 (booster RB51). Cells were incubated at 37°C and 5% CO2 for 7 days and pulsed for 18 h with [3H]thymidine. Means within a sampling time with different letters are significantly different (P < 0.05). The mean response of control bison PBMC incubated in the absence of antigen was 15,415 ± 6,259.
Fig 5
Fig 5
Proliferative responses to 108 CFU of gamma-irradiated RB51 by peripheral blood mononuclear cells from bison inoculated with saline (control) or vaccinated with 1010 CFU of RB51 by dart (dart RB51) or by hand injection (hand RB51). Cells were incubated at 37°C and 5% CO2 for 7 days and pulsed for 18 h with [3H]thymidine. Means within a sampling time with different letters are significantly different (P < 0.05). The mean response of control bison PBMC incubated in the absence of antigen was 5,415 ± 1,265.
Fig 6
Fig 6
Gamma interferon production by peripheral blood mononuclear cells from bison initially vaccinated with saline (control), bison initially vaccinated with 1010 CFU of RB51 (hand RB51 once), or bison initially vaccinated and then booster vaccinated 60 weeks later with 1010 CFU of RB51 (booster RB51). Cells were incubated at 37°C and 5% CO2 for 48 h in the presence or absence of 108 CFU of gamma-irradiated RB51. Results are expressed as the log of the mean net gamma interferon production (production in wells containing RB51 − production in wells without antigen). Means within a sampling time with different letters are significantly different (P < 0.05).
Fig 7
Fig 7
Gamma interferon production by peripheral blood mononuclear cells from bison inoculated with saline (control) or vaccinated with 1010 CFU of RB51 by dart (dart RB51) or by hand injection (hand RB51). Cells were incubated at 37°C and 5% CO2 for 48 h in the presence or absence of 108 CFU of gamma-irradiated RB51. Results are expressed as the log of the mean net gamma interferon production (production in wells containing RB51 − production in wells without antigen). Means within a sampling time with different letters are significantly different (P < 0.05).
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References

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