Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe
- PMID:2227411
- DOI: 10.1101/gad.4.8.1332
Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe
Abstract
The Cks1 protein is a component of the Cdc28 protein kinase in the budding yeast Saccharomyces cerevisiae. This paper reports the cloning of two homologs of the S. cerevisiae CKS1 gene from human cells. These homologs, CKShs1 and CKShs2, both encode proteins of 79 amino acids that share considerable homology at the amino acid level with the products of CKS1 from S. cerevisiae and suc1+ from the fission yeast Schizosaccharomyces pombe. Both human homologs are capable of rescuing a null mutation of the S. cerevisiae CKS1 gene when expressed from the S. cerevisiae GAL1 promoter. S. pombe suc1+ expressed from the GAL1 promoter is also capable of rescuing a S. cerevisiae cks1 null mutation. Ckshs1 or Ckshs2 protein linked to Sepharose beads can bind the Cdc28/Cdc2 protein kinase from both S. cerevisiae and human cells. The CKShs1 and CKShs2 mRNAs are expressed in different patterns through the cell cycle in HeLa cells, which may reflect specialized roles for the encoded proteins.
Similar articles
- Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.Ninomiya-Tsuji J, Nomoto S, Yasuda H, Reed SI, Matsumoto K.Ninomiya-Tsuji J, et al.Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9006-10. doi: 10.1073/pnas.88.20.9006.Proc Natl Acad Sci U S A. 1991.PMID:1717994Free PMC article.
- The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.Hadwiger JA, Wittenberg C, Mendenhall MD, Reed SI.Hadwiger JA, et al.Mol Cell Biol. 1989 May;9(5):2034-41. doi: 10.1128/mcb.9.5.2034-2041.1989.Mol Cell Biol. 1989.PMID:2664468Free PMC article.
- A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.Elledge SJ, Spottswood MR.Elledge SJ, et al.EMBO J. 1991 Sep;10(9):2653-9. doi: 10.1002/j.1460-2075.1991.tb07808.x.EMBO J. 1991.PMID:1714386Free PMC article.
- Suc1: cdc2 affinity reagent or essential cdk adaptor protein?Vogel L, Baratte B.Vogel L, et al.Prog Cell Cycle Res. 1996;2:129-35. doi: 10.1007/978-1-4615-5873-6_13.Prog Cell Cycle Res. 1996.PMID:9552390Review.
- Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Huang Y.Huang Y.Nucleic Acids Res. 2002 Apr 1;30(7):1465-82. doi: 10.1093/nar/30.7.1465.Nucleic Acids Res. 2002.PMID:11917007Free PMC article.Review.
Cited by
- A family of human cdc2-related protein kinases.Meyerson M, Enders GH, Wu CL, Su LK, Gorka C, Nelson C, Harlow E, Tsai LH.Meyerson M, et al.EMBO J. 1992 Aug;11(8):2909-17. doi: 10.1002/j.1460-2075.1992.tb05360.x.EMBO J. 1992.PMID:1639063Free PMC article.
- Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe.Hughes DA, MacNeill SA, Fantes PA.Hughes DA, et al.Mol Gen Genet. 1992 Feb;231(3):401-10. doi: 10.1007/BF00292709.Mol Gen Genet. 1992.PMID:1538696
- Akt1/PKB upregulation leads to vascular smooth muscle cell hypertrophy and polyploidization.Hixon ML, Muro-Cacho C, Wagner MW, Obejero-Paz C, Millie E, Fujio Y, Kureishi Y, Hassold T, Walsh K, Gualberto A.Hixon ML, et al.J Clin Invest. 2000 Oct;106(8):1011-20. doi: 10.1172/JCI8252.J Clin Invest. 2000.PMID:11032861Free PMC article.
- CKS protein overexpression renders tumors susceptible to a chemotherapeutic strategy that protects normal tissues.Tat J, Loriot C, Henze M, Spruck C, Felding BH, Reed SI.Tat J, et al.Oncotarget. 2017 Dec 4;8(70):114911-114923. doi: 10.18632/oncotarget.22931. eCollection 2017 Dec 29.Oncotarget. 2017.PMID:29383129Free PMC article.
- Ectopic expression of cdc2/cdc28 kinase subunit Homo sapiens 1 uncouples cyclin B metabolism from the mitotic spindle cell cycle checkpoint.Hixon ML, Flores AI, Wagner MW, Gualberto A.Hixon ML, et al.Mol Cell Biol. 1998 Nov;18(11):6224-37. doi: 10.1128/MCB.18.11.6224.Mol Cell Biol. 1998.PMID:9774639Free PMC article.
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous